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1.WO/2024/105137AIR-DRIED CELL MONOLAYERS AND METHODS OF PREPARING THE SAME
WO 23.05.2024
国际分类 C12N 5/09
C化学;冶金
12生物化学;啤酒;烈性酒;果汁酒;醋;微生物学;酶学;突变或遗传工程
N微生物或酶;其组合物;繁殖、保藏或维持微生物;变异或遗传工程;培养基。
5未分化的人类、动物或植物细胞,如细胞系;组织;它们的培养或维持;其培养基
09肿瘤细胞
申请号 PCT/EP2023/081979 申请人 ETH ZURICH 发明人 SEVERIN, Yannik
The invention relates to methods for the creation of a cell monolayer comprising: a) providing a cell population; b) culturing said cell population in a liquid medium; and c) air-drying the cell population with a sample evaporator to create a cell monolayer; and optionally d) staining the air-dried sample with histological or other stains. The invention is particularly advantageous for use in high-throughput, high-content analysis of cell samples and, in particular, examining the effect of therapeutic agents on said cell samples.
2.WO/2024/104777T CELL-NK CELL INTERACTION INHIBITORS FOR USE IN DISEASE TREATMENT
WO 23.05.2024
国际分类 C12N 5/0783
C化学;冶金
12生物化学;啤酒;烈性酒;果汁酒;醋;微生物学;酶学;突变或遗传工程
N微生物或酶;其组合物;繁殖、保藏或维持微生物;变异或遗传工程;培养基。
5未分化的人类、动物或植物细胞,如细胞系;组织;它们的培养或维持;其培养基
07动物细胞或组织
071脊椎动物细胞或组织,例如人类细胞或组织
078来自血液或免疫系统的细胞
0783T cells; NK cells; Progenitors of T or NK cells
申请号 PCT/EP2023/080370 申请人 DEUTSCHES KREBSFORSCHUNGSZENTRUM STIFTUNG DES ÖFFENTLICHEN RECHTS 发明人 FRIEDRICH, Mirco
The present invention relates to a T cell-NK cell interaction inhibitor for use in improving in vivo persistence and/or activity of T cells. The present invention also relates to a method for producing a preparation of T cells from a cell population comprising T cells and known or suspected to comprise NK cells, to a method of identifying a subject amenable to treatment with a T cell-NK cell interaction inhibitor, to a method for identifying a T cell-NK cell interaction inhibitor, and to a use of a T cell-NK cell interaction inhibitor for expanding T cells.
3.WO/2024/106532CARBON-DIOXIDE-REDUCING INTERIOR ACCESSORY
WO 23.05.2024
国际分类 C12M 3/00
C化学;冶金
12生物化学;啤酒;烈性酒;果汁酒;醋;微生物学;酶学;突变或遗传工程
M酶学或微生物学装置
3组织、人类、动物或植物细胞或病毒培养装置
申请号 PCT/JP2023/041421 申请人 SHELTER JAPAN CO., LTD. 发明人 YANO Akihiko
Provided is a carbon-dioxide-reducing interior accessory that is easy to maintain and manage and has a high degree of design freedom. A culture vessel 10 in which an algal culture broth L1 is stored has an inner wall provided in a ring shape and an outer wall provided in a ring shape in a region outside the inner wall, an opening also being formed in the culture vessel. The culture vessel 10 also comprises a light source device 20 that irradiates the inner wall with light, the light emitted from the light source device 20 being visible through the inner wall, the algal culture broth L1, and the outer wall. External air EA that is blown into the algal culture broth L1 from an external air introduction nozzle 41, in the process of rising through the algal culture broth L1, reduces carbon dioxide and generates oxygen, which is discharged from the opening, through the effect of photosynthesis by algae present in the algal culture broth L1.
4.WO/2024/107012LACTIPLANTIBACILLUS PLANTARUM STRAIN, CULTURE MEDIUM DERIVED THEREFROM, AND ANTIALLERGIC USES THEREOF
WO 23.05.2024
国际分类 C12N 1/20
C化学;冶金
12生物化学;啤酒;烈性酒;果汁酒;醋;微生物学;酶学;突变或遗传工程
N微生物或酶;其组合物;繁殖、保藏或维持微生物;变异或遗传工程;培养基。
1微生物本身,如原生动物;及其组合物;繁殖、维持或保藏微生物或其组合物的方法;制备或分离含有一种微生物的组合物的方法;及其培养基
20细菌;其培养基
申请号 PCT/KR2023/018563 申请人 GI BIOME INC. 发明人 JANG, Myung Ho
The present invention relates to a novel microorganism, a lysate and culture medium thereof, and an extract of the culture medium, and to antiallergic uses thereof. A novel strain, and a lysate or culture medium derived therefrom, an extract of the culture medium, or a mixture thereof according to one aspect can be usefully employed to prevent, alleviate, or treat allergic diseases.
5.WO/2024/103187FAP2-DERIVED ANTIBODIES AND VACCINES AGAINST FUSOBACTERIUM
WO 23.05.2024
国际分类 C07K 14/195
C化学;冶金
07有机化学
K
14具有多于20个氨基酸的肽;促胃液素;生长激素释放抑制因子;促黑激素;其衍生物
195来自细菌
申请号 PCT/CA2023/051551 申请人 PROVINCIAL HEALTH SERVICES AUTHORITY 发明人 HOLT, Robert
A new composition of matter composed of engineered sequences for the expression of Fap2-derived polypeptides that provoke immunogenic responses against Fusobacterium spp. is provided. Antibodies and vaccines produced using such sequences and methods of use are also provided.
6.WO/2024/103667一种冻干保护剂及粪菌的冻干工艺
WO 23.05.2024
国际分类 C12N 1/04
C化学;冶金
12生物化学;啤酒;烈性酒;果汁酒;醋;微生物学;酶学;突变或遗传工程
N微生物或酶;其组合物;繁殖、保藏或维持微生物;变异或遗传工程;培养基。
1微生物本身,如原生动物;及其组合物;繁殖、维持或保藏微生物或其组合物的方法;制备或分离含有一种微生物的组合物的方法;及其培养基
04保藏或维持活的微生物
申请号 PCT/CN2023/096011 申请人 SHANGHAI BIOTECAN PHARMACEUTICALS CO., LTD. 发明人 WANG, Mengzhu
本申请提供一种冻干保护剂及粪菌的冻干工艺,所述冻干保护剂的制备原料包括糖类物质和高分子保护剂,所述糖类物质包括低聚木糖、海藻糖和甘露糖醇的组合。本申请提供的冻干保护剂及粪菌的冻干工艺可以保护粪便中提取菌液的活性,有效提升粪菌菌群的冻干存活率。
7.WO/2024/107597NOVEL RESISTANCE GENES ASSOCIATED WITH DISEASE RESISTANCE IN SOYBEANS
WO 23.05.2024
国际分类 C07K 14/415
C化学;冶金
07有机化学
K
14具有多于20个氨基酸的肽;促胃液素;生长激素释放抑制因子;促黑激素;其衍生物
415来自植物
申请号 PCT/US2023/079319 申请人 SYNGENTA CROP PROTECTION AG 发明人 LIU, Qingli
Methods and compositions for identifying, selecting and/or producing a pathogen resistant plant, plant cell or seed (e.g., a legume or soybean plant, plant cell or seed) are provided. Polynucleotides and polypeptides are provided, which when expressed in a plant increase disease and/or pathogen resistance (e.g., to Asian Soy Rust) of the plant. Plants, plant cells and seed are provided comprising such polypeptides and polynucleotides, as are methods for their use.
8.WO/2024/107927DUAL-RNA-GUIDED, SPLIT-PEGRNA RECORDER FOR MOLECULAR INTERACTION
WO 23.05.2024
国际分类 C12N 9/12
C化学;冶金
12生物化学;啤酒;烈性酒;果汁酒;醋;微生物学;酶学;突变或遗传工程
N微生物或酶;其组合物;繁殖、保藏或维持微生物;变异或遗传工程;培养基。
9酶,如连接酶(6.);酶原;其组合物;制备、活化、抑制、分离或纯化酶的方法
10转移酶(2.)
12转移含磷基团,如激酶(2.7)
申请号 PCT/US2023/079962 申请人 UNIVERSITY OF WASHINGTON 发明人 CHOI, Junhong
Split-pegRNA recorder reagents and methods are provided that provide specific molecular interactions within cells induce precise genome editing that inserts an event-specific barcode sequence into the predetermined genomic locus of DNA Tape. The split pegRNAs include (a) a crRNA component, including in 5' to 3' order (i) a spacer sequence for a genomic locus of interest; (ii) a crRNA repeat sequence necessary for binding to a CRISPR-Cas protein; and (iii) a first RNA extension; and (b) a petracrRNA component, including in 5' to 3' order (i) a second RNA extension; (ii) a petracrRNA antirepeat sequence necessary for binding to a CRISPR-Cas protein; (iii) a gRNA scaffiald; wherein the crRNA component and the petracrRNA component are not covalently bound to each other.
9.WO/2024/108092PRIME EDITOR DELIVERY BY AAV
WO 23.05.2024
国际分类 C12N 9/12
C化学;冶金
12生物化学;啤酒;烈性酒;果汁酒;醋;微生物学;酶学;突变或遗传工程
N微生物或酶;其组合物;繁殖、保藏或维持微生物;变异或遗传工程;培养基。
9酶,如连接酶(6.);酶原;其组合物;制备、活化、抑制、分离或纯化酶的方法
10转移酶(2.)
12转移含磷基团,如激酶(2.7)
申请号 PCT/US2023/080226 申请人 THE BROAD INSTITUTE, INC. 发明人 LIU, David, R.
Provided herein are methods of delivering "split" prime editors into a cell, e.g., via a recombinant adeno-associated virus (rAAV), to form a complete and functional prime editor. The prime editor may be split into two sections or three sections, with at least two sections being fused with one part of an intein system (e.g., intein-N and intein-C encoded by dnaEn and dnaEc, respectively). Upon co-expression, the two sections of the prime editor are ligated together via intein-mediated protein splicing. Recombinant AAV vectors (z.e., nucleic acid molecules each comprising an intein) and particles for the delivery of the split prime editor, compositions comprising such AAV vectors and particles, and methods of using such rAAV vectors and particles are also provided. Methods of administering such compositions and AAV particles to a subject are further provided.
10.WO/2024/104416抑制SCAP基因表达的siRNA、其缀合物和药物组合物及用途
WO 23.05.2024
国际分类 C12N 15/113
C化学;冶金
12生物化学;啤酒;烈性酒;果汁酒;醋;微生物学;酶学;突变或遗传工程
N微生物或酶;其组合物;繁殖、保藏或维持微生物;变异或遗传工程;培养基。
15突变或遗传工程;遗传工程涉及的DNA或RNA,载体(如质粒)或其分离、制备或纯化;所使用的宿主
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
113调节基因表达的非编码核酸,如反义寡核苷酸
申请号 PCT/CN2023/131980 申请人 BEIJING WINSUNNY PHARMACEUTICAL CO., LTD 发明人 WANG, Yan
本发明涉及抑制固醇调节元件结合蛋白裂解激活蛋白(SCAP)基因表达的siRNA、siRNA缀合物、包含其的药物组合物、及其用途。所述siRNA中的每个核苷酸各自独立地为修饰或未修饰的核苷酸,该siRNA含有正义链和反义链。所述siRNA及其缀合物和药物组合物可以有效治疗和/或预防与SCAP基因过表达相关的疾病。