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1. (WO2015175638) ANALYSE SÉLECTIVE DE MOLÉCULES BIOLOGIQUES MODIFIÉES AVEC DES NANOPORES À L'ÉTAT SOLIDE
Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

CLAIMS

1. A method of biological molecule composition analysis comprising:

providing a mixture comprising biological molecules complexed with a translocating agent and non-complexed biological molecules;

contacting the mixture with a membrane comprising at least one nanopore;

applying an electric field across the nanopore; and

translocating the biological molecules complexed with the translocating agent through the at least one nanopore, wherein translocation of the complexed biological molecules is selectively detected.

2. The method of claim 1 further comprising measuring change in current through the nanopore during one or more translocation events of the complexed biological molecules.

3. The method of claim 2 further comprising measuring the rate of translocation events of the complexed biological molecules.

4. The method of claim 3 further comprising determining concentration of the complexed biological molecules from the rate of translocation events.

5. The method of claim 4, wherein the concentration of the complexed biological molecules exhibits a substantially linear relationship with the rate of translocation events.

6. The method of claim 1 further comprising recovering the translocated complexed biological molecules.

7. The method of claim 1, wherein the translocating agent is a chemical species of sufficient charge to permit selective translocation detection of the complexed biological molecules with the applied electric field set by an applied voltage.

8. The method of claim 1, wherein the complexed biological molecules and non-complexed biological molecules are independently selected from the group consisting of nucleic acids and proteins.

9. The method of claim 1, wherein the translocating agent comprises a single strand nucleic acid modified with an affinity tag for binding one or more molecular species prior to

translocation of the complexed biological molecules.

10. The method of claim 9, wherein the affinity tag binds one or more proteins.

11. The method of claim 10, wherein the affinity tag comprises biotin or a derivative thereof.

12. The method of claim 9, wherein the biological molecules complexed with the

translocating agent are single strand nucleic acids having sequence complimentary to the translocating agent.

13. The method of claim 12, wherein the single strand nucleic acids comprise one or more types of R A.

14. The method of claim 2, wherein the complexed biological molecules are nucleic acids and the rate of the translocation events varies linearly with length of the nucleic acids.

15. The method of claim 1, wherein the at least one nanopore has a diameter of 5 to 30 nm.

16. The method of claim 1, wherein the at least one nanopore has a diameter of 10 to 20 nm.

17. The method of claim 1, wherein a voltage of the applied electric field is 1 mV to 5 V.