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Paramétrages

Paramétrages

1. WO2005063789 - ISOLATION DE BIVITTOSIDE D À PARTIR D'HOLOTHURIE ET ACTIVITÉ DE LADITE SUBSTANCE

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

We claim:
1. A process for the isolation of Bivittoside D from Bohadschia vitiensis, said process comprising soaking B. vitiensis material in a first polar solvent, filtering the material and decanting the solvent followed by soaking the material in a second aqueous polar solvent, extracting the material by filtration, concentrating the extracted material under reduced pressure to obtain a thick viscous crude extract, euting the crude extract followed by crystallization to obtain a pure saponin Bivittoside D.
2. A process as claimed in claim 1 wherein the first polar organic solvent used for extraction is selected from the group consisting of methanol, ethanol, propanol, butanol, water and any mixture thereof.
3. A process as claimed in claim 1 wherein the first polar organic solvent used for extraction is selected from the group consisting of methanol, ethanol, propanol, butanol, water and any mixture thereof.
4. A process as claimed in claim 1 wherein the first polar solvent comprises of n-butanol saturated with water. "
5. A process as claimed in claim 1 wherein the viscous crude extract is eluted using silica gel or gel filtration column and wherein the eluent used is selected from the group consisting of chloroform, methanol, ethanol, water and any mixture thereof.
6. A process as claimed in claim 1 wherein the yield of the bivittoside D is in the range of 25 - 30 %.
7. A spermicidal composition comprising an effective amount of bivittoside D and one or more pharmaceutically acceptable additives.
8. A composition as claimed in claim 7 wherein the bivittoside D is used in an amount of 0.01 % to 1.0% aqueous solution.
9. Use of bivittoside D isolated from Bohadschia vitiensis as a spermicidal agent.
10. Use as claimed in claim 9 wherein the bivittoside D is used as a spermicidal agent in pure form.
11. Use as claimed in claim 9 wherein the bivittoside D is used in an amount of 0.01 % to 1.0% aqueous solution.
12. A composition as claimed in claim 7 wherein the composition is in the form of a cream, jelly, free-flowing powder, solution, suspension, and alcoholic extract.
13. A fungicidal composition comprising a active amount of bivittoside D isolated from Bohadschia vitiensis and one or more conventional additives.

14. A composition as claimed in claim 13 wherein the amount of bivittoside D present in the composition is in the range of 0.39 mg/ml to 12.57 mg/ml.
15. Use of bivittoside D isolated from Bohadschia vitiensis as a fungicidal compound against Candida albicans, Cryptococcus neoformans, Sporothrix schenckii, Aspergillus fumigatus and Frichophyton mentagrophytes pathogens.
16. A composition as claimed in claim 13 wherein the composition is in the form of a cream, jelly, free-flowing powder, solution, suspension, and alcoholic extract.