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Paramétrages

Paramétrages

1. WO2005060396 - COMPOSITIONS NANOTOPOGRAPHIQUES ET PROCEDES D'ORGANISATION DES CELLULES DANS LES STRUCTURES TISSULAIRES RESULTANT DE MANIPULATIONS

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

WE CLAIM

1. A substrate having micromachined surface structures provided thereon, wherein said micromachined surface structures comprise nanotopographic features superimposed thereon, the nanotopographic features being arranged in such a manner so as to organize multiple cell types into desired subassemblies within said micromachined surface structures.

2. The substrate as recited in Claim 1, wherein one or more micromachined surface structures defines the walls and floor of a channel.

3. A substrate as recited in Claim 1, wherein the nanotopographic features facilitate adhesion of one or more cell types.

4. A substrate as recited in Claim 2, wherein the nanotopographic features are oriented to preferentially adhere one or more cell types to a desired location on the substrate.

5. A substrate as recited in Claim 1, wherein the nanotopographic features are oriented to laterally align one or more cell types.

6. A substrate as recited in Claim 1, wherein the nanotopographic features are oriented to form a grid.

7. A substrate as recited in Claim 1, wherein the nanotopographic features are generated by a lithographic technique.

8. A substrate as recited in Claim 1, wherein the cell types are selected from the group consisting of endothelial cells, smooth or skeletal muscle cells, myocytes, cardiac cells fibroblasts, chondrocytes, adipocytes, fibromyoblasts, ductile cells, skin cells, hepatocytes, kidney cells, pancreatic islet cells, intestinal cells, osteoblasts, hematopoietic cells and stem cells.

9. A method of organizing cells on a substrate for use in engineering tissue, said method comprising the steps of:
a) generating a micromachined surface structures; and
b) superimposing nanotopographic features on the micromachined surface structures in such a manner so as to organize multiple cell types into desired subassemblies for use in constructing engineered tissue.

10. The method according to Claim 9, wherein the micromachined surface structure
defines the walls and floor of a channel.

11. A method according to Claim 9, wherein the step of superimposing nanotopographic features includes arranging the nanotopographic features in predefined locations on the substrate.

12. A method according to Claim 9, wherein the step of superimposing nanotopographic features includes orienting the nanotopographic features to
preferentially adhere one or more cell types to a desired location on the substrate.

13. A tissue engineered system comprising one or more layers, wherein each layer includes micromachined surface structures having nanotopographic features superimposed thereon, the nanotopographic features being arranged in such a manner so as to organize multiple cell types into desired subassemblies within said micromachined surface structures.

14. The system according to Claim 13, wherein a semi-permeable membrane is positioned between the layers.

15. The system of claim 13, wherein one or more micromachined surface structures defines the walls and floor of a channel.

16. The system according to Claim 15, wherein the channels are divided longitudinally into two compartments by a centrally positioned membrane, and wherein each compartment comprises a different cell type.

17. The. system according to Claim 13, further comprising a pumping means for circulating fluid through the system.

18. The system according to Claim 13, further comprising nutrient supply and excretion removal lines in fluid communication with the system.

19. The system according to Claim 13, wherein the nanotopographic features facilitate adhesion of one or more cell types.

20. The system according to Claim 19, wherein the nanotopographic features are oriented to preferentially adhere one or more cell types to a desired location on a layer.

21. The system according to Claim 13, wherein the nanotopographic features are oriented to laterally align one or more cell types.

22. The system according to Claim 13, wherein the nanotopographic features are oriented to form a grid.

23. The system according to Claim 13, wherein the nanotopographic features are generated by a lithographic technique.

24. The system according to Claim 13, wherein the cell types are selected from the group consisting of endothelial cells, smooth or skeletal muscle cells, myocytes, cardiac cells, fibroblasts, chondrocytes, adipocytes, fibromyoblasts, ductile cells, skin cells, hepatocytes, kidney cells, pancreatic islet cells, intestinal cells, osteoblasts, hematopoietic cells and stem cells.