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1. WO2001072955 - REGULATION DE LA PROTEINE HUMAINE APPARENTEE A NEDD1

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

[ EN ]

CLALMS

1. An isolated polynucleotide encoding a NEDD 1 -related polypeptide and being selected from the group consisting of:

a) a polynucleotide encoding a NEDDl-related polypeptide comprising an amino acid sequence selected from the group consisting of:

amino acid sequences which are at least about 50% identical to
the amino acid sequence shown in SEQ ID NO. 2; and
the amino acid sequence shown in SEQ ID NO. 2.

b) a polynucleotide comprising the sequence of SEQ ID NO. 1 ;

c) a polynucleotide which hybridizes under stringent conditions to a polynucleotide specified in (a) and (b);

d) a polynucleotide the sequence of which deviates from the polynucleotide sequences specified in (a) to (c) due to the degeneration of the genetic code; and

e) a polynucleotide which represents a fragment, derivative or allelic variation of a polynucleotide sequence specified in (a) to (d).

2. An expression vector containing any polynucleotide of claim 1.

3. A host cell containing the expression vector of claim 2.

4. A substantially purified NEDDl-related polypeptide encoded by a polynucleotide of claim 1.

5. A method for producing a NEDDl-related polypeptide, wherein the method comprises the following steps:

a) culturing the host cell of claim 3 under conditions suitable for the expression of the NEDD 1 -related polypeptide; and

b) recovering the NEDDl-related polypeptide from the host cell culture.

6. A method for detection of a polynucleotide encoding a NEDDl-related polypetide in a biological sample comprising the following steps:

a) hybridizing any polynucleotide of claim 1 to a nucleic acid material of a biological sample, thereby forming a hybridization complex; and

b) detecting said hybridization complex.

7. The method of claim 6, wherein before hybridization, the nucleic acid material of the biological sample is amplified.

8. A method for the detection of a polynucleotide of claim 1 or a NEDDl- related polypeptide of claim 5 comprising the steps of contacting a biological sample with a reagent which specifically interacts with the polynucleotide or the NEDDl-related polypeptide.

9. A diagnostic kit for conducting the method of any one of claims 6 to 8.

10. A method of screening for agents which decrease the activity of a NEDDl- related protein, comprising the steps of:

contacting a test compound with any NEDDl-related polypeptide encoded by any polynucleotide of claim 1;

detecting binding of the test compound of the NEDDl-related polypeptide, wherein a test compound which binds to the polypeptide is identified as a potential therapeutic agent for decreasing the activity of a NEDDl-related protein.

11. A method of screening for agents which regulate the activity of a NEDDl- related protein, comprising the steps of:

contacting a test compound with a NEDDl-related polypeptide encoded by any polynucleotide of claim 1; and

detecting a NEDDl-related activity of the polypeptide, wherein a test compound which increases the NEDDl-related activity is identified as a potential therapeutic agent for increasing the activity of the NEDDl-related protein, and wherein a test compound which decreases the NEDDl-related activity of the polypeptide is identified as a potential therapeutic agent for decreasing the activity of the NEDDl-related protein.

12. A method of screening for agents which decrease the activity of a NEDDl- related protein, comprising the steps of:

contacting a test compound with any polynucleotide of claim 1 and

detecting binding of the test compound to the polynucleotide, wherein a test compound which binds to the polynucleotide is identified as a potential therapeutic agent for decreasing the activity of NEDDl-related protein.

13. A method of reducing the activity of NEDDl-related protein, comprising the steps of: contacting a cell with a reagent which specifically binds to any polynucleotide of claim 1 or any NEDDl-related polypeptide of claim 4, whereby the activity of NEDDl-related protein is reduced.

14. A reagent that modulates the activity of a NEDDl-related polypeptide or a polynucleotide wherein said reagent is identified by the method of any of the claims 10 to 12.

15. A pharmaceutical composition, comprising:
the expression vector of claim 2 or the reagent of claim 14 and a pharmaceutically acceptable carrier.

16. Use of the pharmaceutical composition of claim 15 for modulating the activity of a NEDDl-related protein in a disease.

17. Use of claim 16 wherein the disease includes over-proliferation of cells of the central and peripheral nervous system.