Traitement en cours

Veuillez attendre...

Paramétrages

Paramétrages

Aller à Demande

1. WO1999003326 - SYSTEME DE TRANSFORMATION PAR LE POLLEN, A L'AIDE DE MILIEUX SOLIDES

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

[ EN ]

We claim :
1. A method for producing a transgenic plant
comprising:

a. obtaining pollen from a first plant,

b. applying a lawn of Agrobacteria to a solid
pollen culture medium, the Agrobacteria
comprising at least one heterologous gene
sequence capable of being transferred to a
plant cell,

c. applying the pollen to the solid medium,

d. allowing the pollen to germinate and grow on
the medium, thereby producing transgenic
pollen,

e. applying the transgenic pollen to the stigma of a second plant capable of being fertilized by
the pollen of the first plant, thereby
fertilizing the second plant,

f. obtaining transgenic seed from the second
plant,

g. germinating the transgenic seed to obtain a
transgenic plant .

2. The method of claim 1, wherein the first and second plants are selected from the group consisting of tobacco plants, cotton plants and soybean plants.

3. The method of claim 1, wherein the solid pollen culture medium comprises 10% agarose, 25% sucrose, 0.52 mM KN03, 3.06 mM MnS04, 1.66 mM H3B03, 0.42 mM MgS04'7H20 and 1.0 μM A3 gibberellic acid.

4. The method of claim 2, wherein the plants are dry stigma pollen plants.

5. The method of claim 4, when the plants are cotton plants .

6. The method of claim 5, wherein the second cotton plant is an emasculated cotton plant.

7. The method of claim 1, comprising the additional step of emasculating the second plant prior to the application of the transgenic pollen thereto.

8. A medium for pollen germination and pollen tube growth comprising agarose, sucrose, KN03, MnS04, H3B03, MgS04, and gibberellic acid.