Recherche dans les collections de brevets nationales et internationales
Certains contenus de cette application ne sont pas disponibles pour le moment.
Si cette situation persiste, veuillez nous contacter àObservations et contact
1. (WO1995033846) APPAREIL DE REACTION A PUCE RENFERMANT UN GROUPEMENT DE SONDES BIOLOGIQUES ET SA FABRICATION
Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

WHAT IS CLAIMED IS:
1. A method of making probe chips comprising the steps of:
forming a plurality of probe arrays on a substrate;
separating said substrate into a plurality of chips, each of said chips comprising at least one probe array thereon; and
mating at least one of said chips to a package, said package comprising a reaction chamber, said reaction chamber comprising inlets for flowing fluid therein, said at least one probe array in fluid communication with said reaction chamber.

2. The method as recited in claim l wherein said package is made by the steps of :
injection molding first and second halves of said package; and
mating said first and second halves together.

3. The method as recited in claim 2 wherein one of said halves comprises flow channels therein, said flow channels in communication with said inlets.

4. The method as recited in claim 3 further comprising the step of applying a reenterable seal to flow channels in said package.

5. The method as recited in claim 1 wherein said substrate comprises alignment marks for forming said probe arrays thereon in a desired position, and wherein said alignment marks are used to identify locations for said separating of said substrate into chips.

6. The method as recited in claim 1 wherein said package comprises an alignment structure thereon, wherein said step of mating said chip to said package uses said alignment structures to position said package at a desired position.

7. The method as recited in claim 1 wherein said package comprises an alignment structure thereon, and further comprising the step of identifying the location of at least one target on said probe array in a scanner, wherein said package is placed at a desired location in said scanner using said alignment structure.

8. The method as recited in claim 1 wherein said step of forming a plurality of probe arrays comprises the steps of :
selectively exposing said substrate to light;
coupling selected monomers to said substrate where said substrate has been exposed to light.

9. The method as recited in claim 1 wherein said step of separating comprises the steps of:
scribing said substrate in desired locations;
breaking said substrate along said scribe lines.

10. The method as recited in claim 1 wherein said step of forming a plurality of probe arrays on said substrate is a step of forming a plurality of oligonucleotide probe arrays on said substrate .

11. The method as recited in claim 10 further comprising the steps of flowing labeled oligonucleotide target molecules through said reaction chamber and identifying where said target molecules have bound to said substrate.

12. The method as recited in claim 11 wherein said package comprises a temperature probe and further comprising the step of monitoring and adjusting a temperature in said reaction chamber.

13. The method as recited in claim 1 wherein said package is formed by the steps of:
forming first and second package portions; and
acoustically welding said first and second package portions together.

14. The method as recited in claim 1 wherein said step of mating said chips to packages comprises the step of binding said chips to said package with an adhesive.

15. The method as recited in claim 14 wherein said packages comprise a recessed region thereon, whereby said chips do not extend above a surface of said packages.

16. The method as recited in claim 1 further comprising the step of flowing target molecules through said reaction chamber.

17. An apparatus for packaging a substrate, said apparatus comprising:
a substrate having a first surface and a second surface, said first surface comprising a probe array;
a body having a mounting surface with a fluid cavity, said second surface attached to said cavity; and
a cover attached to said mounting surface for sealing said cavity.

18. The apparatus of claim 17 wherein said cavity comprises an inlet port and an outlet port, said inlet and outlet ports permitting fluids to circulate into and through said cavity

19. The apparatus of claim 18 wherein said inlet and outlet ports comprise a reenterable seal .

20. The apparatus of claim 17 wherein said probe array comprises an array of oligonucleotide probes

21. An apparatus for packaging a substrate, said apparatus comprising:
a substrate having a first surface and a second surface, said first surface comprising a probe array and said second surface being an outer periphery of said first surface;
a body having a mounting surface, an upper surface, and a cavity bounded by said mounting surface and said upper surface, said second surface being attached to said cavity and said first surface being within said cavity; and
a cover attached to said mounting surface for defining an upper boundary to said cavity;
wherein said cavity comprises a diffuser and a
concentrator, said diffuser and said concentrator permitting laminar fluid flow through said cavity.

22. The apparatus of claim 21 wherein said probe array comprises an array of oligonucleotide probes.

23. The apparatus of claim 21 wherein said cover comprises a depression for receiving a temperature control element to maintain a reaction temperature in said cavity.

24. The apparatus of claim 21 wherein said cover comprises a first half mated to a second half.

25. The apparatus of claim 24 wherein said first half comprises a first channel and a second channel, said first channel being in fluid communication with said diffuser and said second channel being in fluid communication with said concentrator.

26. The apparatus of claim 25 wherein said second half comprises a third channel and a fourth channel, said third channel being in fluid communication with said first channel, and said fourth channel being in fluid communication with said second channel.

27. The apparatus of claim 26 wherein said first channel and said second channel comprise reenterable seals for sealing fluid in said cavity.