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1. (WO1990007006) EXPRESSION DE LA PROTEINE DU CIRCUMSPOROZOITE DE $i(PLASMODIUM) DANS DES CELLULES D'INSECTE
Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

What is claimed is:
1. A Plasmodium CS protein gene expression unit comprising a DNA coding sequence for said protein and a regulatory element necessary for transcription of the coding sequence and translation within an insect cell.
2. The gene expression unit of claim 1 which codes for a P. falciparum CS protein.
3. The gene expression unit of claim 2 which codes for a protein lacking the carboxy terminal anchor sequence, or lacking a signal sequence, or lacking both a signal and an anchor sequence.
4. The gene expression unit of claim 3 in which the regulatory element functions in Drosophila cells.
5. The gene expression unit of claim 3 in which the regulatory element functions in Lepidoptera cells.
6. The gene expression unit of claim 4 wherein said regulatory element comprises an actin 5C promoter or a Drosophila metallothionein promoter.
7. The gene expression unit of claim 6 as present in pMTcsp391, pMTcsp398, or pMTcsp412.
8. The gene expression unit of claim 5 in which the regulatory element functions in Spodoptera frugiperda cells.
9. The gene expression unit of claim 8 wherein said regulatory element comprises the AcMNPV polyhedrin gene promoter.
10. A DNA vector comprising the gene expressio unit of claim 1.
11. A recombinant baculovirus comprising the gene expression unit of claim 1.
12. An insect cell transfected with the vector of claim 10.
13. The insect cell of claim 12 which is a
Drosophila cell.
14. An insect cell transfected with the
recombinant baculovirus of claim 11.

15. The insect cell of claim 14 which is a
Lepidoptera cell.
16. The insect cell of claim 15 which is S.
frugiperda.
17. A P. falciparum CS protein produced in insect cells.
18. The protein of claim 17 which is the full length CS protein.
19. The protein of claim 17 which is lacking the ° carboxy terminal anchor region, or lacking a signal peptide, or lacking both a signal peptide and a carboxy terminal anchor region.
20. A protein produced in insect cells, which is encoded by the gene expression unit of claim 1.
21. A protein produced in insect cells, which is encoded by the gene expression unit of claim 3.
22. A vaccine for stimulating protection against malarial infection wherein such vaccine comprises an immunoprotective and non-toxic quantity of the protein encoded by the CS gene expression unit of claim 1.
23. A method for protecting humans against disease symptoms associated with malarial infections which comprises administering to humans a safe and effective amount of the vaccine of claim 22.
24. A method for production of a Plasmodium CS protein in insect cells comprising culturing in a suitable medium insect cells transfected with a Plasmodium CS protein gene expression unit, said cells being capable of expressing said protein.
25. The method of claim 24 wherein the insect cells are Drosophila cells.
26. The method of claim 25 wherein said cells are transfected with a first vector containing the coding sequence for hygromycin B phosphotransferase and a second vector containing the coding sequence for a Plasmodium CS protein gene expression unit.

27. The method of claim 26 wherein said first vector is pCOHYGRO.
28. The method of claim 26 wherein said second vector comprises the CS DNA coding sequence of pMTcsp391, pMTcsp398 or pMTcsp412.
29. The method of claim 25 wherein the
Drosophila cells are D. melanoqaster S2 cells.
30. The method of claim 29 wherein said S2 cells are co-transfected with the vector pCOHYGRO and a vector comprising a CS gene expression unit as is present in pMTcsp391, pMTcsp398 or pMTcsp412.
31. The method of claim 24 wherein the insect cells are Lepidoptera cells.
32. The method of claim 31 wherein said cells are co-transfected with a first vector comprising
baculovirus DNA and a second vector comprising plasmid DNA which contains the coding sequence for a Plasmodium CS gene expression unit.
33. The method of claim 31 wherein the
Lepidoptera cells are S. frugiperda.
34. A vaccine for stimulating protection against malarial infection wherein such vaccine comprises an immunoprotective and non-toxic quantity of the CS protein produced by the method of claim 24.
35. The protein of claim 20 for use in the manufacture of a vaccine for stimulating protection against malarial infection.