(EN) PURPOSE: A removal of protein aggregates from a biopharmaceutical formulation in a flow-through mode is provided to effectively isolate target monomeric proteins from protein aggregates. CONSTITUTION: A flow-through chromatography method for isolating target monomeric proteins from protein aggregates in a sample comprises the steps of making a sample and a solid support come in contact. The solid support contains one or more cationic exchange binding groups which are attached in a concentration of approximately 1-30 mM and is selectively conjugated with the protein aggregates. The solid support is selected among a chromatography resin, a membrane, a porous bead, a porous monolith, a winged fiber, a fabric, and a non-woven fabric. The protein aggregates are lower order protein aggregates. COPYRIGHT KIPO 2013 null [Reference numerals] (AA) MAb polymer; (BB) MAb monomer; (CC) CEX surface of the conventional technique; (DD) Low ligand density surface of this invention
(KO) 본 발명은 샘플로부터 단백질 응집물을 플로우 쓰루 모드로 제거하기 위한 신규 조성물과 방법을 제공한다.
