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PROBLEM TO BE SOLVED: To provide an antifreeze liquid and a vitrification liquid useful as a solution for storing biological materials at low temperature, or the like.
SOLUTION: There are provided an antifreeze liquid manufactured by dissolving an overcooling promoter containing catechin type tannin of 0.01 g/L to 30 g/L in water or a solution containing an additive depending on applications, and a vitrification liquid which is a solution containing the overcooling promoter containing catechin type tannin of 0.01 to 30 g/L, and a freezing damage inhibitor of 20 to 90 vol.% alone or in a combination of them, with the balance (10 to 80 vol.%) being water or the solution containing an additive depending on applications. An example of catechin type tannin includes low molecule polyphenol derived from litchi fruit, polyphenol derived from litchi fruit (LFP) and polyphenol derived from grape seed (GSP).
SELECTED DRAWING: None
COPYRIGHT: (C)2017,JPO&INPIT
【課題】生物材料を低温で保存するための溶液等として有用な不凍性液体及びガラス化液の提供。【解決手段】カテキン型タンニンを含む過冷却促進剤0.01g/L〜30g/Lを水または用途に応じた添加物を含む水溶液に溶解させて調製される不凍性液体、及びタンニンを含む過冷却促進剤を0.01〜30g/L含み、凍害防止剤を単独または組み合わせて20〜90容積%、残余(10〜80容積%)が水または用途に応じた添加物を含む水溶液であるガラス化液。カテキン型タンニンとしては、ライチフルーツ由来低分子化ポリフェノール、ライチフルーツ由来ポリフェノール(LFP)、及びブドウ種子由来ポリフェノール(GSP)が挙げられる。【選択図】なし