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1. JP2013189427 - REMOVAL OF PROTEIN AGGREGATE FROM BIOPHARMACEUTICAL PREPARATION IN FLOW-THROUGH MODE

Office
Japon
Numéro de la demande 2013027361
Date de la demande 15.02.2013
Numéro de publication 2013189427
Date de publication 26.09.2013
Numéro de délivrance 5768070
Date de délivrance 26.06.2015
Type de publication B2
CIB
C07K 1/16
CCHIMIE; MÉTALLURGIE
07CHIMIE ORGANIQUE
KPEPTIDES
1Procédés généraux de préparation de peptides
14Extraction; Séparation; Purification
16par chromatographie
CPC
B01D 15/362
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
15Separating processes involving the treatment of liquids with solid sorbents
08Selective adsorption, e.g. chromatography
26characterised by the separation mechanism
36involving ionic interaction
361Ion-exchange
362Cation-exchange
C07K 1/165
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General methods for the preparation of peptides ; , i.e. processes for the organic chemical preparation of peptides or proteins of any length
14Extraction; Separation; Purification
16by chromatography
165mixed-mode chromatography
C07K 1/18
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General methods for the preparation of peptides ; , i.e. processes for the organic chemical preparation of peptides or proteins of any length
14Extraction; Separation; Purification
16by chromatography
18Ion-exchange chromatography
B01D 15/363
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
15Separating processes involving the treatment of liquids with solid sorbents
08Selective adsorption, e.g. chromatography
26characterised by the separation mechanism
36involving ionic interaction
361Ion-exchange
363Anion-exchange
B01D 15/3809
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
15Separating processes involving the treatment of liquids with solid sorbents
08Selective adsorption, e.g. chromatography
26characterised by the separation mechanism
38involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
3804Affinity chromatography
3809of the antigen-antibody type, e.g. protein A, G, L chromatography
B01J 39/20
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
39Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
08Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
16Organic material
18Macromolecular compounds
20obtained by reactions only involving unsaturated carbon-to-carbon bonds
Déposants EMD MILLIPORE CORP
メルク パテント ゲーエムベーハー
Inventeurs KOZLOV MIKHAIL
ミハイル・コズロフ
CATALDO WILLIAM
ウイリアム・カタルド
POTTY AJISH
アジシユ・ポテイ
GALIPEAU KEVIN
ケビン・ガリポー
ジエームズ・ハムジク
ホアキン・ウマーナ
ラルス・ピーク
Mandataires 特許業務法人川口國際特許事務所
Données relatives à la priorité 61/609,533 12.03.2012 US
61/666,578 29.06.2012 US
Titre
(EN) REMOVAL OF PROTEIN AGGREGATE FROM BIOPHARMACEUTICAL PREPARATION IN FLOW-THROUGH MODE
(JA) フロースルー式での生物製剤からのタンパク質凝集体の除去
Abrégé
(EN)

PROBLEM TO BE SOLVED: To provide novel compositions and methods for removal of protein aggregates from a sample in a flow-through mode.

SOLUTION: A flow-through chromatography method of separating a monomeric protein of interest from protein aggregates in a sample includes a step of contacting the sample with a solid support comprising one or more cation exchange binding groups attached thereto, at a density of about 1 to about 30 mM, wherein the solid support selectively binds protein aggregates, thereby separating the monomeric protein of interest from protein aggregates.

COPYRIGHT: (C)2013,JPO&INPIT


(JA)

【課題】フロースルー式で試料からタンパク質凝集体を除去する新規の組成物および方法を提供する。
【解決手段】試料中のタンパク質凝集体から対象の単量体タンパク質を分離するフロースルークロマトグラフィー方法であって、試料を、約1−約30mMの密度で1つまたは複数の陽イオン交換結合基が結合した固体担体と接触させるステップを含み、固体担体がタンパク質凝集体に選択的に結合し、それにより、タンパク質凝集体から対象の単量体タンパク質を分離する、フロースルークロマトグラフィー方法。
【選択図】図2A