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1. JP2010051297 - METHOD FOR MODIFYING SUGAR CHAIN STRUCTURE IN PLANT, AND PLANT

Office Japon
Numéro de la demande 2008222961
Date de la demande 30.08.2008
Numéro de publication 2010051297
Date de publication 11.03.2010
Numéro de délivrance 5435535
Date de délivrance 20.12.2013
Type de publication B2
CIB
A01H 1/00
ANÉCESSITÉS COURANTES DE LA VIE
01AGRICULTURE; SYLVICULTURE; ÉLEVAGE; CHASSE; PIÉGEAGE; PÊCHE
HNOUVEAUTÉS VÉGÉTALES OU PROCÉDÉS POUR LEUR OBTENTION; REPRODUCTION DE PLANTES PAR DES TECHNIQUES DE CULTURE DE TISSUS
1Procédés de modification des génotypes
C07K 14/415
CCHIMIE; MÉTALLURGIE
07CHIMIE ORGANIQUE
KPEPTIDES
14Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés
415provenant de végétaux
C12N 5/10
CCHIMIE; MÉTALLURGIE
12BIOCHIMIE; BIÈRE; SPIRITUEUX; VIN; VINAIGRE; MICROBIOLOGIE; ENZYMOLOGIE; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE
NMICRO-ORGANISMES OU ENZYMES; COMPOSITIONS LES CONTENANT; CULTURE OU CONSERVATION DE MICRO-ORGANISMES; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE; MILIEUX DE CULTURE
5Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
10Cellules modifiées par l'introduction de matériel génétique étranger, p.ex. cellules transformées par des virus
C12N 15/09
CCHIMIE; MÉTALLURGIE
12BIOCHIMIE; BIÈRE; SPIRITUEUX; VIN; VINAIGRE; MICROBIOLOGIE; ENZYMOLOGIE; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE
NMICRO-ORGANISMES OU ENZYMES; COMPOSITIONS LES CONTENANT; CULTURE OU CONSERVATION DE MICRO-ORGANISMES; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE; MILIEUX DE CULTURE
15Techniques de mutation ou génie génétique; ADN ou ARN concernant le génie génétique, vecteurs, p.ex. plasmides, ou leur isolement, leur préparation ou leur purification; Utilisation d'hôtes pour ceux-ci
09Technologie d'ADN recombinant
C12P 21/00
CCHIMIE; MÉTALLURGIE
12BIOCHIMIE; BIÈRE; SPIRITUEUX; VIN; VINAIGRE; MICROBIOLOGIE; ENZYMOLOGIE; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE
PPROCÉDÉS DE FERMENTATION OU PROCÉDÉS UTILISANT DES ENZYMES POUR LA SYNTHÈSE D'UN COMPOSÉ CHIMIQUE DONNÉ OU D'UNE COMPOSITION DONNÉE, OU POUR LA SÉPARATION D'ISOMÈRES OPTIQUES À PARTIR D'UN MÉLANGE RACÉMIQUE
21Préparation de peptides ou de protéines
CPC
C12N 15/8218
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
C12N 9/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
C12N 15/8245
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8242with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
8243involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
8245involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
C12P 21/005
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
005Glycopeptides, glycoproteins
Déposants NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE & TECHNOLOGY
国立研究開発法人産業技術総合研究所
Inventeurs MATSUO YUKITAKE
松尾 幸毅
MATSUMURA TAKESHI
松村 健
Titre
(EN) METHOD FOR MODIFYING SUGAR CHAIN STRUCTURE IN PLANT, AND PLANT
(JA) 植物における糖鎖構造の改変方法及びその植物体
Abrégé
(EN)

PROBLEM TO BE SOLVED: To provide a method for reducing or inhibiting the modification of an N-linked sugar chain with fucose in a plant, a transgenic plant in which the fucose modification is reduced or inhibited, and others.

SOLUTION: The amount of GDP-fucose in a plant cell is reduced by inhibiting the transcription/translation of a gene encoding an enzyme involved in the synthesis of a sugar nucleotide GDP-fucose through posttranscriptional gene silencing (PTGS), virus-induced gene silencing (VIGS) or transcriptional gene silencing (TGS). In this manner, the modification of a sugar chain such as a glycoprotein sugar chain, a glycolipid sugar chain, an oligosaccharide or a polysaccharide with fucose is reduced or inhibited, thereby producing a glycoprotein or the like in which a fucose-modified part, which can act as an allergen, is removed. It becomes possible to use a medical glycoprotein produced in a plant for clinical purposes.

COPYRIGHT: (C)2010,JPO&INPIT

(JA)

【課題】植物におけるN−結合型糖鎖へのフコース修飾を減少、抑制させる方法、当該フコース修飾を減少、抑制した植物形質転換体等を提供する。
【解決手段】糖ヌクレオチドGDP−フコースの合成に関与する酵素をコードする遺伝子の転写、翻訳を、posttranscriptional gene silencing(PTGS)、ウイルス誘導ジーンサイレンシング(VIGS)、又はtranscriptional gene silencing(TGS)により阻害し、植物細胞内におけるGDP−フコース量を減少させることにより、糖蛋白質糖鎖、糖脂質糖鎖、オリゴ糖、多糖等を含む糖鎖へのフコース修飾を減少・抑制させる。
【効果】アレルゲンとなり得るフコース修飾が除去された糖蛋白質等の生産が可能となり、植物で生産させた医療用糖蛋白質の臨床応用が可能となる。
【選択図】図1

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