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1. ES2541223 - Plataforma de alto rendimiento para cribados subcelulares in vivo en larvas de vertebrados

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Office
Espagne
Numéro de la demande 11701928
Date de la demande 25.01.2011
Numéro de publication 2541223
Date de publication 16.07.2015
Type de publication T3
CIB
C12M 1/26
CCHIMIE; MÉTALLURGIE
12BIOCHIMIE; BIÈRE; SPIRITUEUX; VIN; VINAIGRE; MICROBIOLOGIE; ENZYMOLOGIE; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE
MAPPAREILLAGE POUR L'ENZYMOLOGIE OU LA MICROBIOLOGIE
1Appareillage pour l'enzymologie ou la microbiologie
26Inoculateur ou échantillonneur
C12M 3/00
CCHIMIE; MÉTALLURGIE
12BIOCHIMIE; BIÈRE; SPIRITUEUX; VIN; VINAIGRE; MICROBIOLOGIE; ENZYMOLOGIE; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE
MAPPAREILLAGE POUR L'ENZYMOLOGIE OU LA MICROBIOLOGIE
3Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
CPC
G01N 33/5088
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
5005involving human or animal cells
5008for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
5082Supracellular entities, e.g. tissue, organisms
5088of vertebrates
C40B 30/06
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
30Methods of screening libraries
06by measuring effects on living organisms, tissues or cells
C40B 60/10
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
60Apparatus specially adapted for use in combinatorial chemistry or with libraries
10for identifying library members
G01N 1/14
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
02Devices for withdrawing samples
10in the liquid or fluent state
14Suction devices, e.g. pumps; Ejector devices
G01N 1/28
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
G01N 15/1475
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation, e.g. flow cytometers
1468with spatial resolution of the texture or inner structure of the particle
1475using image analysis for extracting features of the particle
Déposants Massachusetts Institute of Technology
Inventeurs YANIK, Mehmet F.
WASSERMAN, Steven C.
PARDO, Carlos
CHANG, Tsung-Yao
GILLELAND, Cody L.
Mandataires Ungría Ungría Javier
Données relatives à la priorité 12713263 26.02.2010 US
Titre
(ES) Plataforma de alto rendimiento para cribados subcelulares in vivo en larvas de vertebrados
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Abrégé
(EN) High throughput system for in vivo screens on vertebrate larvae. The system includes a source of vertebrate larvae in a liquid medium and loading tube means for aspirating a larva. A detector assembly is provided to differentiate passage of a larva from bubbles and/or debris. An imaging means is provided for both confocal imaging and wide-field fluorescence imaging of the larva. A laser is provided for optical manipulation of the larva.
(FR) La présente invention a pour objet un système à haut débit pour cribles in vivo sur des larves de vertébrés. Le système comprend une source de larves de vertébrés dans un milieu liquide et des moyens de tube de chargement pour aspirer une larve. Un ensemble détecteur est prévu pour différencier le passage d'une larve et celui de bulles et/ou de débris. Un moyen d'imagerie est prévu à la fois pour l'imagerie confocale et l'imagerie par fluorescence à large champ de la larve. Un laser est prévu pour la manipulation optique de la larve.
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