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1. CN1263434 - Pollen-based transformation system using solid media

Office Chine
Numéro de la demande 98807068.5
Date de la demande 14.07.1998
Numéro de publication 1263434
Date de publication 16.08.2000
Type de publication A
CIB
A01H 1/06
ANÉCESSITÉS COURANTES DE LA VIE
01AGRICULTURE; SYLVICULTURE; ÉLEVAGE; CHASSE; PIÉGEAGE; PÊCHE
HNOUVEAUTÉS VÉGÉTALES OU PROCÉDÉS POUR LEUR OBTENTION; REPRODUCTION DE PLANTES PAR DES TECHNIQUES DE CULTURE DE TISSUS
1Procédés de modification des génotypes
06Procédés de mutation, p.ex. traitements par produits chimiques ou irradiations
CPC
A01H 1/04
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1Processes for modifying genotypes
04Processes of selection
Déposants United States of America, as Represented by the Secretary of Agriculture
由农业部长代表的美利坚合众国
Inventeurs John J. Burke
约翰·J·伯克
Melven J. Oliver
梅尔文·J·奥利弗
Jeffrey P. Velten
杰弗里·P·费尔藤
Mandataires lin xiaogong
永新专利商标代理有限公司
Données relatives à la priorité 08892735 15.07.1997 US
Titre
(EN) Pollen-based transformation system using solid media
(ZH) 使用固体培养基的基于花粉的转化系统
Abrégé
(EN)
A medium is disclosed that supports pollen germination and pollen tube growth in the presence of Agrobacterium, the medium comprising agarose, sucrose, NO3, MnSO4, H3BO3, MgSO4 and gibberellic acid. A method is disclosed for the genetic transformation of plants and lines by a pollen-based Agrobacterim-mediated transformation. The method comprises the steps of obtaining pollen from a first plant, applying a lawn of Agrobacteria to a solid pollen culture medium, the Agrobacterium comprising at least one heterologous gene sequence capable of being transferred to a plant cell, applying the pollen to the solid medium, allowing the pollen to germinate and grow on the medium, whereby the Agrobacterium attaches to the pollen tube or mediates transfer of the heterologous gene sequence to the germinating pollen to obtain transgenic pollen, applying the transgenic pollen to the stigma of a second plant capable of being fertilized by the pollen of the first plant, thereby fertilizing the second plant, obtaining transgenic seed from the second plant and germinating the transgenic seed to obtain a transgenic plant. The method is particularly suited for use with so-called ''dry stigma'' pollen.

(ZH)

本发明公开了一种在农杆菌的存在下支持花粉萌发和花粉管生长的培养基,所述的培养基包括琼脂糖、蔗糖、NO3;、MnSO4、H3BO3、MgSO4和赤霉酸。还公开了用基于花粉的农杆菌介导的转化技术对植物和品系进行遗传转化的方法,该方法包括从第一个植物中获得花粉,将一片农杆菌菌苔加到固体花粉培养基上,该农杆菌包括至少一种能被转移至植物细胞中的异源基因序列,将花粉加到该固体培养基上,使花粉在培养基上萌发并生长,从而农杆菌附着于花粉管或者介导所述的异源基因序列转移至萌发的花粉中而获得转基因花粉,将转基因花粉加至能被第一个植物的花粉受精的第二个植物的柱头上,从而使第二个植物受精,从第二个植物中获得转基因种子,并萌发转基因种子获得转基因植物。该方法特别适于使用所谓的“干柱头”花粉。