(EN) Disclosed is an in-vitro method to determine efficacy of a cosmetic composition or of one or more ingredients comprised therein to inhibit a particulate pollutant from contacting skin, said method comprising the steps of: (i) contacting a human skin equivalent with a cosmetic composition to form, upon drying, a layer thereof extending along mutually perpendicular X and Y and Z axis where along said Z axis said layer is 1 to 100 [mu]m, where said Z axis indicates thickness of said layer; (ii) depositing, on said layer, a known amount of a model fine particulate matter comprising a first substance responsive to depth profile analysis where said cosmetic composition comprises a second substance, not comprised in said model fine particulate matter but also responsive to said depth profile analysis where the response of said first and second substances are distinguishable; (iii) for a pre-determined period after depositing said model fine particulate matter, periodically measuring responseof said first and said second substance by said depth profile analysis, to thereby determine the amount of said model fine particulate matter at defined intervals along said Z axis; and, (iv) ascertaining said efficacy based on the amount of said model fine particulate matter at said defined intervals along said Z axis throughout said pre-determined period.
(ZH) 公开确定化妆品组合物或包含的一种或多种成分抑制颗粒污染物接触皮肤的功效的体外方法，包括步骤：(i)使人体皮肤等效物与化妆品组合物接触以在干燥后形成沿相互垂直的X、Y和Z轴延伸的层，其中该层沿Z轴为1至100μm，其中Z轴表示层的厚度；(ii)在层上沉积已知量的包含响应深度剖面分析的第一物质的模型微细颗粒物质，其中化妆品组合物包含未包含在模型微细颗粒物质中但也响应深度剖面分析的第二物质，其中第一和第二物质的响应可区分；(iii)在沉积模型微细颗粒物质的预定时间段内，通过深度剖面分析定期测量第一和第二物质的响应从而确定模型微细颗粒物质在沿Z轴的规定间隔处的量；和(iv)基于在整个预定时间段内在沿着Z轴的规定间隔处模型微细颗粒物质的量来确定所述功效。
