(EN)
To form a layer (11) separating two volumes of aqueous solution, especially a
biolayer lipid membrane (BLM)
there is used an apparatus comprising elements defining a chamber (7), the
elements including a body (2) of non-conductive
material having formed therein at least one recess (5) opening into the
chamber, the recess containing an electrode (21). A
pre-treatment coating of a hydrophobic fluid is applied to the body across the
recess. Aqueous solution, having amphiphilic molecules
added thereto, is flowed across the body to cover the recess so that aqueous
solution is introduced into the recess from the
chamber and a layer of the amphiphilic molecules forms across the recess
separating a volume of aqueous solution introduced into the
recess from the remaining volume of aqueous solution.