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1. (CA2990881) REGIONS NON TRADUITES (UTR) AUGMENTANT L'EFFICACITE DE TRADUCTION DES MOLECULES D'ARN

Office : Canada
Numéro de la demande : 2990881 Date de la demande :
Numéro de publication : 2990881 Date de publication : 05.01.2017
Type de publication : A1
Référence PCT: Numéro de la demande :PCTEP2016065297 ; Numéro de publication : Cliquer pour voir les données
CIB :
C12N 15/67
C CHIMIE; MÉTALLURGIE
12
BIOCHIMIE; BIÈRE; SPIRITUEUX; VIN; VINAIGRE; MICROBIOLOGIE; ENZYMOLOGIE; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE
N
MICRO-ORGANISMES OU ENZYMES; COMPOSITIONS LES CONTENANT; CULTURE OU CONSERVATION DE MICRO-ORGANISMES; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE; MILIEUX DE CULTURE
15
Techniques de mutation ou génie génétique; ADN ou ARN concernant le génie génétique, vecteurs, p.ex. plasmides, ou leur isolement, leur préparation ou leur purification; Utilisation d'hôtes pour ceux-ci
09
Technologie d'ADN recombinant
63
Introduction de matériel génétique étranger utilisant des vecteurs; Vecteurs: Utilisation d'hôtes pour ceux-ci; Régulation de l'expression
67
Méthodes générales pour favoriser l'expression
Déposants : ETHRIS GMBH
Inventeurs :
Données relatives à la priorité : 15174683.1 30.06.2015 EP
Titre : (EN) UTRS INCREASING THE TRANSLATION EFFICIENCY OF RNA MOLECULES
(FR) REGIONS NON TRADUITES (UTR) AUGMENTANT L'EFFICACITE DE TRADUCTION DES MOLECULES D'ARN
Abrégé : front page image
(EN) Described is an RNA molecule comprising (a) a coding region coding for a polypeptide; and (b) upstream of said coding region one or more UTR(s) comprising the sequence as shown in SEQ ID NO:1 or a sequence which shows 1 to 4 substitutions in comparison to SEQ ID NO:1 and which results in an RNA molecule having the same or a higher translation efficiency as an RNA molecule comprising an UTR comprising SEQ ID NO:1; and/or (c) downstream of said coding region one or more UTR(s) comprising the sequence as shown in SEQ ID NO:2 or a sequence which shows 1 to 7 substitutions in comparison to SEQ ID NO:2 and which results in an RNA molecule having the same or a higher translation efficiency as an RNA molecule comprising an UTR comprising SEQ ID NO:2; wherein said polypeptide encoded by said coding region is not a cytochrome b-245 alpha polypeptide (CYBA). Moreover, described is a nucleic acid molecule encoding the RNA molecule according to the present invention. Further, described is a vector comprising the nucleic acid molecule according to the present invention and to a host cell comprising the vector according to the present invention. Further, described is a pharmaceutical composition comprising the RNA molecule according to the present invention and optionally a pharmaceutically acceptable carrier. Moreover, described is a kit comprising the RNA molecule according to the present invention. Finally, described is the use of one or more UTR(s) as defined in (b) and/or one or more UTR(s) as defined in (c) for increasing the efficiency of translating a coding region of an RNA molecule into a polypeptide or a protein encoded by said coding region.


Également publié sous:
EP3112469AU2016287436IN201717045893KR1020180035789EP3317415DK3112469
ES2667644CN107849574JP2018519830BR112017028205US20190194669WO/2017/001554