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1. AU1998079088 - Family 6 endo-1,4-beta-glucanase variants and cleaning composit ions containing them

Office Australie
Numéro de la demande 79088/98
Date de la demande 02.07.1998
Numéro de publication 1998079088
Date de publication 18.03.1999
Type de publication A
CIB
C12N 9/42
CCHIMIE; MÉTALLURGIE
12BIOCHIMIE; BIÈRE; SPIRITUEUX; VIN; VINAIGRE; MICROBIOLOGIE; ENZYMOLOGIE; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE
NMICRO-ORGANISMES OU ENZYMES; COMPOSITIONS LES CONTENANT; CULTURE OU CONSERVATION DE MICRO-ORGANISMES; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE; MILIEUX DE CULTURE
9Enzymes, p.ex. ligases (6.); Proenzymes; Compositions les contenant; Procédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
14Hydrolases (3.)
24agissant sur les composés glycosyliques (3.2)
42agissant sur les liaisons bêta-glucosidiques-1, 4, p.ex. cellulase
C11D 3/386
CCHIMIE; MÉTALLURGIE
11HUILES, GRAISSES, MATIÈRES GRASSES OU CIRES ANIMALES OU VÉGÉTALES; LEURS ACIDES GRAS; DÉTERGENTS; BOUGIES
DCOMPOSITIONS DÉTERGENTES; EMPLOI D'UNE SUBSTANCE, UTILISÉE SEULE, COMME DÉTERGENT; SAVON OU FABRICATION DU SAVON; SAVONS DE RÉSINE; RÉCUPÉRATION DE LA GLYCÉRINE
3Autres composés entrant dans les compositions détergentes couvertes par le groupe C11D1/110
16Composés organiques
38Produits de composition non exactement définie
386Préparations contenant des enzymes
CPC
C12N 9/2437
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
24acting on glycosyl compounds (3.2)
2402hydrolysing O- and S- glycosyl compounds (3.2.1)
2405Glucanases
2434acting on beta-1,4-glucosidic bonds
2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
C11D 3/38645
CCHEMISTRY; METALLURGY
11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
DDETERGENT COMPOSITIONS
3Other compounding ingredients of detergent compositions covered in group C11D1/00
16Organic compounds
38Products with no well-defined composition ; , e.g. natural products
386Preparations containing enzymes ; , e.g. protease, amylase
38645containing cellulase
C12Y 302/01004
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
302Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
Déposants Novo Nordisk A/S
Inventeurs Andersen, Kim Vilbour
Damgaard, Bo
Lund, Henrik
Nielsen, Jack Bech
Schulein, Martin
Données relatives à la priorité 813/97 04.07.1997 DK
Titre
(EN) Family 6 endo-1,4-beta-glucanase variants and cleaning composit ions containing them
Abrégé
(EN)
Cleaning compositions comprising one or more enzymes having cellulolytic activity wherein at least 25 % of the total weight of cellulolytic active protein derives from the presence of a $i(Humicola) endo-1,4-$g(b)-glucanase or $i(Humicola)-like cellulase of the glycolsyl hydrolase family 6, the $i(Humicola)-like cellulase being an enzyme comprising a catalytically core domain having an amino acid sequence being at least 35 % homologous to the appended SEQ ID NO:4; a method of constructing a variant of a parent $i(Humicola) family 6 (Cel6B) endo-beta-1,4-glucanase or a $i(Humicola)-like family 6 cellulase which variant has endo-beta-1,4-glucanase activity and improved detergent compatibility as compared to the parent endo-beta-1,4-glucanase or cellulase; and variants mutated eg. in positions 20, 56, 94, 95, 103, 182, 183 and 318 (Cel6B numbering).

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