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An electrostatic spray disinfecting system is integrated into a mobile environment to enable the mobile environment to be automatically subjected to an electrostatic spraying operation when the mobile environment is moving or stationary without the need for a human operator to participate in the spraying operation. An electrostatic sprayer of the system is mechanically coupled to a structure of the mobile environment in a preselected position relative to an internal compartment of the mobile environment. A reservoir of disinfecting material and is fluidly coupled to the electrostatic sprayer for delivering a flow of the disinfecting material to the sprayer. When an electrical switching circuit is placed in an On state, electrical power is delivered to the electrostatic sprayer to electrostatically charge the particles of the disinfecting material delivered to the electrostatic sprayer and to cause the electrostatically-charged particles to be sprayed from the electrostatic spray nozzle into the internal compartment.