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1. (WO2018227664) METHODS FOR EXTRACTING AND PURIFYING NOSTOC SPHAEROIDES KUTZING PHYCOBILIPROTEIN, AND PURIFIED PHYCOERYTHRIN
국제사무국에 기록된 최신 서지정보    정보 제출

공개번호: WO/2018/227664 국제출원번호: PCT/CN2017/090808
공개일: 20.12.2018 국제출원일: 29.06.2017
IPC:
C07K 14/405 (2006.01) ,C07K 1/36 (2006.01) ,C07K 1/18 (2006.01) ,C07K 1/34 (2006.01) ,C07K 1/14 (2006.01)
C SECTION C — 화학; 야금
07
유기화학
K
펩티드 (Peptides)
14
20이상의 아미노산을 갖는 펩티드; 가스트린(Gastrins); 소마토스타틴(Somatostatins); 멜라노트로핀(Melanotropins); 그 유도체
405
.조류(algae)로부터 유도된 것
C SECTION C — 화학; 야금
07
유기화학
K
펩티드 (Peptides)
1
펩티드의 일반적인 제조
14
추출; 분리; 정제
36
상이한 2가지 또는 그 이상의 공정의 결합에 의한 것
C SECTION C — 화학; 야금
07
유기화학
K
펩티드 (Peptides)
1
펩티드의 일반적인 제조
14
추출; 분리; 정제
16
크로마토그래피에 의한 것
18
이온교환 크로마토그래피
C SECTION C — 화학; 야금
07
유기화학
K
펩티드 (Peptides)
1
펩티드의 일반적인 제조
14
추출; 분리; 정제
34
여과, 한외여과(ultrafiltration) 또는 역삼투에 의한 것
C SECTION C — 화학; 야금
07
유기화학
K
펩티드 (Peptides)
1
펩티드의 일반적인 제조
14
추출; 분리; 정제
출원인:
湖南炎帝生物工程有限公司 HUNAN YANDI BIOENGINEERING CO., LTD. [CN/CN]; 中国湖南省株洲市 国家高新技术开发区(天元区)明日路8号 No.8, Mingri Road, National Hi-tech Development Zone (Tianyuan District) Zhuzhou, Hunan 412007, CN
발명자:
余佳 YU, Jia; CN
张瑞华 ZHANG, Ruihua; CN
王玉兰 WANG, Yulan; CN
占豪 ZHAN, Hao; CN
陈盛 CHEN, Sheng; CN
王维 WANG, Wei; CN
대리인:
上海麦其知识产权代理事务所(普通合伙) MSJ LAW OFFICE; 中国上海市 普陀区中山北路3663号358幢301B室董红曼 Maggie Dong Room 301B, Building 358, No.3663, North Zhongshan Road, Putuo District Shanghai 200062, CN
우선권 정보:
201710447906.114.06.2017CN
발명의 명칭: (EN) METHODS FOR EXTRACTING AND PURIFYING NOSTOC SPHAEROIDES KUTZING PHYCOBILIPROTEIN, AND PURIFIED PHYCOERYTHRIN
(FR) PROCÉDÉS D'EXTRACTION ET DE PURIFICATION DE LA PHYCOBILIPROTÉINE DE NOSTOC SPHAEROIDES KÜTZING ET PHYCOÉRYTHRINE PURIFIÉE
(ZH) 一种葛仙米藻胆蛋白的提取、纯化方法及纯化的藻红蛋白
요약서:
(EN) Provided is a method for purifying a Nostoc sphaeroides Kutzing phycoerythrin, the method comprising: (1) selecting an anion exchange column for being passed through; (2) formulating a PB buffer solution, i.e., buffer A, and a PB-NaCl buffer solution, i.e., buffer B; (3) dissolving a coarsely extracted Nostoc sphaeroides Kutzing phycobiliprotein powder with the PB buffer solution, i.e., buffer A, and subjecting same to centrifugation and filtration; (4) washing a pump, and balancing the ion exchange column with the PB buffer solution, i.e., buffer A; (5) directly loading the Nostoc sphaeroides Kutzing phycobiliprotein dissolved in the PB buffer solution, i.e., buffer A, and prepared in step (3), carrying out gradient elution with the PB-NaCl buffer solution, i.e., buffer B, and collecting an eluent; (6) cleaning the ion exchange column with a salt solution, and then reversely rinsing same with a base solution; and (7) subjecting the eluent to ultrafiltration concentration and dialysis to obtain a purified Nostoc sphaeroides Kutzing phycoerythrin aqueous solution. In addition, further provided is a method for extracting a Nostoc sphaeroides Kutzing phycobiliprotein.
(FR) La présente invention concerne un procédé de purification d'une phycoérythrine de Nostoc sphaeroides Kützing, le procédé consistant à : (1) sélectionner une colonne échangeuse d'anions pour la faire passer à travers ; (2) formuler une solution tampon PB, c'est-à-dire le tampon A, et une solution tampon PB-NaCl, c'est-à-dire, le tampon B ; (3) dissoudre une poudre de phycobiliprotéine de Nostoc sphaeroides Kützing grossièrement extraite avec la solution tampon PB, c'est-à-dire le tampon A, et la soumettre à une centrifugation et à une filtration ; (4) laver une pompe et équilibrer la colonne échangeuse d'ions avec la solution tampon PB, c'est-à-dire, le tampon A ; (5) charger directement la phycobiliprotéine de Nostoc sphaeroides Kützing dissoute dans la solution tampon PB, c'est-à-dire le tampon A et préparée à l'étape (3), réaliser une élution par gradient avec la solution tampon PB-NaCl, c'est-à-dire le tampon B et collecter un éluant ; (6) nettoyer la colonne échangeuse d'ions avec une solution saline, puis effectuer un rinçage inverse de celle-ci avec une solution de base ; et (7) soumettre l'éluant à une concentration par ultrafiltration et à une dialyse pour obtenir une solution aqueuse de phycoérythrine de Nostoc sphaeroides Kützing purifiée. De plus, l'invention concerne en outre un procédé d'extraction de la phycobiliprotéine de Nostoc sphaeroides Kützing.
(ZH) 提供了一种葛仙米藻红蛋白的纯化方法,包括:(1)选用阴离子交换柱过柱;(2)配置PB缓冲液BufferA和PB-NaCl缓冲液Buffer B;(3)葛仙米藻胆蛋白粗提粉末用PB缓冲液BufferA溶解,离心,过滤;(4)洗泵,用PB缓冲液BufferA平衡离子交换柱;(5)将步骤(3)制备的溶解于PB缓冲液BufferA中的葛仙米藻胆蛋白直接上样,PB-NaCl缓冲液Buffer B梯度洗脱,收集洗脱液;(6)用盐溶液清洗离子交换柱,然后用碱溶液反向冲洗;(7)对淋洗液进行超滤浓缩,透析,得到纯化的葛仙米藻红蛋白水溶液。此外,还提供了一种葛仙米藻胆蛋白的提取方法。
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지정국: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
아프리카지역 지식재산권기구(ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
유라시아 특허청 (AM, AZ, BY, KG, KZ, RU, TJ, TM)
유럽 특허청(EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
공개언어: 중국어 (ZH)
출원언어: 중국어 (ZH)