국제 및 국내 특허문헌 검색

1. (WO2018032464) HIGH-THROUGHPUT CELL LINE SCREENING METHOD FOR CHO-DHFR EXPRESSION SYSTEM

Pub. No.:    WO/2018/032464    International Application No.:    PCT/CN2016/095877
Publication Date: Fri Feb 23 00:59:59 CET 2018 International Filing Date: Fri Aug 19 01:59:59 CEST 2016
IPC: C12N 15/85
C12N 5/10
C12Q 1/02
Applicants: SUNSHINE LAKE PHARMA CO., LTD.
广东东阳光药业有限公司
Inventors: LIU, Jing
刘静
YANG, Bin
杨彬
ZHANG, Caixia
张彩霞
CHEN, Hualin
陈华林
CHEN, Li
陈莉
SUEN, Wen-Chen
孙文正
LI, Wenjia
李文佳
Title: HIGH-THROUGHPUT CELL LINE SCREENING METHOD FOR CHO-DHFR EXPRESSION SYSTEM
Abstract:
A high-throughput cell line screening method for a CHO-Dhfr expression system. The method comprises: performing three rounds of methotrexate stress treatment, selecting a cell pool with 40-50% cell viability, and performing monoclonal conversion in a specific semi-solid nutrient medium.