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1. (WO1989012679) METHOD FOR PRODUCING THERMALLY STABLE ALPHA-AMYLASE
국제사무국에 기록된 최신 서지정보   

공개번호:    WO/1989/012679    국제출원번호:    PCT/US1989/002683
공개일: 28.12.1989 국제출원일: 19.06.1989
IPC:
C12N 9/28 (2006.01)
출원인: GREAT LAKES CHEMICAL CORPORATION [US/US]; P.O. Box 2200, Highway 52, N.W., West Lafayette, IN 47906 (US)
발명자: CHEN, James, C.; (US).
LAMM, William, R.; (US).
RACINE, Francis, M.; (US).
TURNER, Hal, R.; (US)
대리인: VITTUM, Daniel, W., Jr. @; Kirkland & Ellis, 200 East Randolph Drive, Suite 6100, Chicago, IL 60601 (US)
우선권 정보:
209,426 21.06.1988 US
발명의 명칭: (EN) METHOD FOR PRODUCING THERMALLY STABLE ALPHA-AMYLASE
(FR) PROCEDE DE PRODUCTION D'ALPHA-AMYLASE THERMIQUEMENT STABLE
요약서: front page image
(EN)A process for the production of thermally stable $i(alpha-amylase) comprising the steps of: cultivating $i(B. licheniformis) ATCC 53,722 in a nutrient medium which contains as a primary carbon source a carbohydrate which normally represses alpha-amylase synthesis, selected from the group consisting of dextrose, starch hydrolysate, glycerol, xylose, mannose, sucrose and mixtures thereof; feeding said primary carbon source to said medium during cultivation; maintaining the level of carbon dioxide evolution from the medium during cultivation between about 4.5 % and 6.5 % of the off-gas when the air flow is 0.5 to 1.0 vvm by controlling the rate at which the primary carbon source is fed to said medium during cultivation; and maintaining the pH of the medium between 6.5 and 7.0.
(FR)Un procédé de production d'$i(alpha-amylase) thermiquement stable comprend les étapes suivantes: la culture de $i(B. licheniformis), ATCC 53.722, dans un milieu nutritif qui contient comme source primaire de carbone un hydrate de carbone qui normalement inhibe la synthèse d'alpha-amylase, sélectionné dans le groupe formé par dextrose, hydrolysat d'amidon, glycérol, xylose, sucrose et leurs mélanges; l'adjonction de cette source primaire de carbone au milieu nutritif pendant la culture; le maintien du niveau de dioxyde de carbone dégagé par le milieu pendant la culture entre 4,5 % et 6,5 % des effluents gazeux lorsque le débit d'air est compris entre 0,5 et 1,0 vvm en ajustant le taux d'adjonction de la source primaire de carbone au milieu pendant la culture; et le maintien du pH du milieu nutritif entre 6,5 et 7,0.
지정국: BG, BR, DK, FI, HU, JP, KR, SU.
European Patent Office (AT, BE, CH, DE, FR, GB, IT, LU, NL, SE).
공개언어: English (EN)
출원언어: English (EN)