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1. (WO2017060122) METHODS OF PRESERVING THE BIOLOGICAL ACTIVITY OF RIBONUCLEIC ACIDS

Pub. No.:    WO/2017/060122    International Application No.:    PCT/EP2016/072927
Publication Date: Fri Apr 14 01:59:59 CEST 2017 International Filing Date: Wed Sep 28 01:59:59 CEST 2016
IPC: A01N 63/02
C12N 15/113
C12N 15/10
Applicants: SYNGENTA PARTICIPATIONS AG
DEVGEN NV
Inventors: FELDMANN, Pascale
FOWLER, Jeffrey, David
MADDELEIN, Wendy
MAILLET, Isabelle
CROMHEECKE, Nina
Title: METHODS OF PRESERVING THE BIOLOGICAL ACTIVITY OF RIBONUCLEIC ACIDS
Abstract:
The present invention provides a method of substantially retaining or otherwise preserving the biological activity of a dsRNA, present in a cell lysate, to post-transcriptionally silence the expression of a gene in a target organism, comprising the step of adding to the lysate a compound having the function of a protein – or amine - cross linking agent. The invention also comprises compositions comprising the lysate comprising dsRNA, and protein cross linking agents, as well as the use of said agents in the method.