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A fluorescent substrate includes a substrate, a fluorescent layer disposed on the substrate, the fluorescent layer emitting fluorescence owing to excitation light passing through an incident surface of the fluorescent layer facing the substrate and emitting the fluorescence through an exit surface of the fluorescent layer facing the incident surface, and reflective portions, which face the incident surface and side surfaces of the fluorescent layer in contact with the incident surface. The reflective portions include a first reflective portion that reflects the excitation light and the fluorescence and a second reflective portion disposed on at least part of the incident surface, the second reflective portion allowing at least light having a peak wavelength of the excitation light to pass through and reflecting at least light having a peak wavelength of the fluorescence.