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1. US20110318759 - Method for assaying keratan sulfate, assay kit therefor and method for detecting joint disease by using the same

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Claims

1. A method for immunologically determining a keratan sulfate level, comprising the steps of:(a): contacting a biological sample, simultaneously or sequentially, with a solid phase on which an anti-keratan sulfate monoclonal antibody is immobilized, and with a labeled anti-keratan sulfate monoclonal antibody, to thereby form, on the solid phase, an immune complex of the anti-keratan sulfate antibodies and the keratan sulfate contained in the biological sample;
(b): detecting a signal produced by the labeled antibody in said immune complex; and
(c): determining the level of keratan sulfate in said biological sample based on the intensity of said detected signal,
wherein said immobilized antibody is BCD-4 and wherein said labeled antibody is labeled MK-172,
wherein the biological sample is derived from a rabbit, guinea pig, rat, mouse or human.
2. The keratan sulfate immunological determination method according to claim 1, wherein the biological sample is derived from a rabbit, guinea pig, rat, or mouse.
3. The keratan sulfate immunological determination method according to claim 1, wherein the biological sample is derived from a human.
4. The keratan sulfate immunological determination method according to claim 1, wherein the biological sample is a blood sample or synovial fluid.
5. The keratan sulfate immunological determination method according to claim 1, wherein the biological sample has been treated with a protease.
6. The keratan sulfate immunological determination method according to claim 5, wherein the protease is one or more members selected from the group consisting of pronase, subtilisin, papain, and trypsin.
7. A keratan sulfate assay kit for use in a keratan sulfate immunological determination method as recited in claim 3, wherein said kit contains BCD-4 and labeled MK-172 antibodies.