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1. US20110318759 - Method for assaying keratan sulfate, assay kit therefor and method for detecting joint disease by using the same

官庁 アメリカ合衆国
出願番号 13142262
出願日 24.12.2009
公開番号 20110318759
公開日 29.12.2011
特許番号 08822229
特許付与日 02.09.2014
公報種別 B2
IPC
G01N 33/564
G物理学
01測定;試験
N材料の化学的または物理的性質の決定による材料の調査または分析
33グループG01N1/00~G01N31/00に包含されない,特有な方法による材料の調査または分析
48生物学的材料,例.血液,尿;血球計
50生物学的材料,例.血液,尿,の化学分析;生物学的特異性を有する配位子結合方法を含む試験;免疫学的試験
53免疫分析;生物学的特異的結合分析;そのための物質
564あらかじめ存在する免疫複合体または自己免疫疾患のためのもの
CPC
G01N 33/53
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
G01N 2400/40
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2400Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
10Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
38Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence, e.g. gluco- or galactomannans, e.g. Konjac gum, Locust bean gum, Guar gum
40Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides
G01N 2800/102
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2800Detection or diagnosis of diseases
10Musculoskeletal or connective tissue disorders
101Diffuse connective tissue disease, e.g. Sjögren, Wegener's granulomatosis
102Arthritis; Rheumatoid arthritis, i.e. inflammation of peripheral joints
出願人 Wakitani Shigeyuki
Seikagaku Corporation
Fujita Hiroshi
Ishimaru Takeshi
Yamamoto Koji
Kurahashi Yasuhiro
Onaya Junichi
Masuda Hiroyuki
発明者 Wakitani Shigeyuki
Fujita Hiroshi
Ishimaru Takeshi
Yamamoto Koji
Kurahashi Yasuhiro
Onaya Junichi
Masuda Hiroyuki
代理人 Sughrue Mion, PLLC
優先権情報 2008-332642 26.12.2008 JP
2009-185244 07.08.2009 JP
発明の名称
(EN) Method for assaying keratan sulfate, assay kit therefor and method for detecting joint disease by using the same
要約
(EN)

The inventions provides a method for immunologically determining a keratan sulfate level which method includes bringing an anti-keratan sulfate monoclonal antibody into contact with a biological sample, the anti-keratan sulfate monoclonal antibody exhibiting a relative reaction specificity between keratan sulfate-I and keratan sulfate-II represented by IC50KS-I/KS-II of 0.4 to 5, to thereby provide a signal; and detecting keratan sulfate contained in the biological sample from the signal. On the basis of the method, the invention also provides a joint disease detection method and a method for assessing the effect of a remedy for a joint disease and a candidate substance therefor. Through these methods, a very small amount of keratan sulfate contained in a sample, can be determined. Particularly, these methods can determine, at high-sensitivity and high-specificity, the total keratan sulfate including keratan sulfate-I, which have been difficult to determine through a conventional technique. The methods also enables detect a joint disease and assess the effect of a remedy for a joint disease or a candidate substance therefor.