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1. KR1020100074220 - METHOD OF MODIFYING ISOELECTRIC POINT OF ANTIBODY VIA AMINO ACID SUBSTITUTION IN CDR

官庁 大韓民国
出願番号 1020107008915
出願日 26.09.2008
公開番号 1020100074220
公開日 01.07.2010
特許番号
特許付与日 08.12.2015
公報種別 B1
IPC
C07K 16/28
C化学;冶金
07有機化学
Kペプチド
16免疫グロブリン,例.モノクローナル抗体またはポリクローナル抗体
18動物またはヒトからの物質に対するもの
28レセプター,細胞表面抗原または細胞表面決定因子に対するもの
A61K 39/39
A生活必需品
61医学または獣医学;衛生学
K医薬用,歯科用又は化粧用製剤
39抗原または抗体を含有する医薬品製剤
39免疫促進付加物によって特徴づけられたもの,例.化学的アジュバント
C07K 1/16
C化学;冶金
07有機化学
Kペプチド
1ペプチドの製造のための一般方法
14抽出;分離;精製
16クロマトグラフィーによるもの
C12N 15/63
C化学;冶金
12生化学;ビール;酒精;ぶどう酒;酢;微生物学;酵素学;突然変異または遺伝子工学
N微生物または酵素;その組成物;微生物の増殖,保存,維持;突然変異または遺伝子工学;培地
15突然変異または遺伝子工学;遺伝子工学に関するDNAまたはRNA,ベクター,例.プラスミド,またはその分離,製造または精製;そのための宿主の使用
09組換えDNA技術
63ベクターを用いた外来遺伝物質の導入;ベクター;そのための宿主の使用;発現の制御
CPC
C07K 16/28
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
18against material from animals or humans
28against receptors, cell surface antigens or cell surface determinants
C12N 15/63
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C07K 1/16
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General methods for the preparation of peptides ; , i.e. processes for the organic chemical preparation of peptides or proteins of any length
14Extraction; Separation; Purification
16by chromatography
A61K 39/39
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
39characterised by the immunostimulating additives, e.g. chemical adjuvants
出願人 CHUGAI SEIYAKU KABUSHIKIKAISHA
추가이 세이야쿠 가부시키가이샤
発明者 IGAWA TOMOYUKI
TSUNODA HIROYUKI
TACHIBANA TATSUHIKO
이가와 도모유키
KURAMOCHI TAICHI
츠노다 히로유키
다치바나 다츠히코
구라모치 다이치
代理人 서종완
優先権情報 JP-P-2007-250165 26.09.2007 JP
JP-P-2007-256063 28.09.2007 JP
発明の名称
(EN) METHOD OF MODIFYING ISOELECTRIC POINT OF ANTIBODY VIA AMINO ACID SUBSTITUTION IN CDR
(KO) CDR의 아미노산 치환에 의해 항체의 등전점을 개변하는 방법
要約
(EN)
It is intended to provide a method of modifying the isoelectric point of an antibody while sustaining the antigen binding activity thereof which comprises modifying the charge of at least one amino acid residue which can be exposed on the surface of the complementarity determining region (CDR). Further, it is intended to provide a method of purifying a multispecific antibody by modifying the isoelectric point thereof and a method of improving the drug kinetics in plasma of an antibody having a modified isoelectric point. Furthermore, it is intended to provide an antibody having a modified isoelectric point, a medicinal composition comprising the antibody as the active ingredient and a method of producing the same. COPYRIGHT KIPO WIPO 2010

(KO)
본 발명자들은, 상보성 결정영역(CDR)의 표면에 노출 가능한 하나 이상의 아미노산 잔기의 전하를 개변함으로써, 항체의 항원으로의 결합활성을 유지하면서 등전점을 개변하는 방법을 제공한다. 본 발명은 또한, 등전점의 개변에 의해 다중 특이성 항체를 정제하는 방법 및 등전점의 개변에 의해 항체의 혈장 중 약물동태를 개선하는 방법을 제공한다. 또한 본 발명은, 등전점이 개변된 항체, 당해 항체를 유효성분으로서 함유하는 의약 조성물 및 그의 제조방법을 제공한다.