(EN) The present invention provides a production method for bacterial cells that, in a multi-stage liquid cultivation process for bacteria, includes a step in which, in the stage prior to the final stage,the bacteria are cultivated using a liquid medium containing an antibiotic substance, and in the final stage, the bacteria are cultivated upon reducing the concentration of the antibiotic substance tobelow that of the prior stage. It is thereby possible to produce the bacterial cells with excellent yield while suppressing the appearance of mutant individuals forming aberrant colonies in the bacteria obtained after cultivation.
(ZH) 本发明提供细菌菌体的制备方法，所述方法包括：在细菌的多阶段的液体培养工序中，在比最终阶段早的阶段用含有抗生素的液体培养基培养细菌，在最终阶段使抗生素的浓度低于该早的阶段而进行培养的工序。由此可以抑制培养后得到的细菌中形成异常菌落的突变个体的出现，并以良好的生产率制备细菌菌体。
