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1. CN111032861 - METHOD FOR GRADUAL CONSTRUCTION OF REASSORTANT INFLUENZA VIRUS

官庁
中華人民共和国
出願番号 201880054431.2
出願日 23.08.2018
公開番号 111032861
公開日 17.04.2020
公報種別 A
IPC
C12N 7/01
C化学;冶金
12生化学;ビール;酒精;ぶどう酒;酢;微生物学;酵素学;突然変異または遺伝子工学
N微生物または酵素;その組成物;微生物の増殖,保存,維持;突然変異または遺伝子工学;培地
7ウイルス,例.バクテリオファージ;それを含む組成物;その調製または精製
01外来遺伝物質の導入によって修飾されたウイルス,例.バクテリオファージ
CPC
C12N 7/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
C12N 2760/16051
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2760ssRNA Viruses negative-sense
00011ssRNA Viruses negative-sense
16011Orthomyxoviridae
16051Methods of production or purification of viral material
C12N 2760/16151
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2760ssRNA Viruses negative-sense
00011ssRNA Viruses negative-sense
16011Orthomyxoviridae
16111Influenzavirus A, i.e. influenza A virus
16151Methods of production or purification of viral material
C12N 2760/16251
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2760ssRNA Viruses negative-sense
00011ssRNA Viruses negative-sense
16011Orthomyxoviridae
16211Influenzavirus B, i.e. influenza B virus
16251Methods of production or purification of viral material
出願人 THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIV
一般财团法人阪大微生物病研究会
発明者 FUJIMOTO TAKAO
藤本贵男
代理人 北京聿宏知识产权代理有限公司 11372
北京聿宏知识产权代理有限公司 11372
優先権情報 2017-163114 28.08.2017 JP
発明の名称
(EN) METHOD FOR GRADUAL CONSTRUCTION OF REASSORTANT INFLUENZA VIRUS
(ZH) 重配流感病毒的阶段性制备方法
要約
(EN) Provided is a method for constructing a reassortant influenza virus that has genomic segments of at least two types of influenza virus when an antigen strain and a donor strain have analogous antigenicity. The method relies upon a step for using (1) a first influenza virus containing an antigen protein (x), (2) a second influenza virus containing an antigen protein (x') having antigenicity analogous to that of strain (1), and (3) a third influenza virus containing an antigen protein (y) having antigenicity different from that of strain (1) to infect hosts with strain (2) and strain (3) and co-culture to construct reassortant influenza viruses, and selecting an influenza virus (Y) comprising the antigen protein (y) from among said viruses, followed by infecting hosts with strain (1) and theselected strain (Y), co-culturing, and selecting an influenza virus (X) comprising the antigen protein (x) from the reassortant influenza viruses constructed from strain (1) and strain (Y).
(ZH) 在抗原株与供体株具有类似的抗原性的情况下,提供一种具有两种以上的流感病毒的基因组片段的重配流感病毒的制备方法。使用包含抗原蛋白质(x)的第一流感病毒(1)、具有与株(1)类似的抗原性的抗原蛋白质(x’)的第二流感病毒(2)、具有与株(1)不同的抗原性的抗原蛋白质(y)的第三流感病毒(3)、且包含以下工序:使宿主感染株(2)和株(3)并进行共培养来制备重配流感病毒并从该病毒中选择具有抗原蛋白质(y)的流感病毒(Y),接着使宿主感染株(1)和上述选择出的株(Y)并进行共培养,并从由株(1)和株(Y)制备的重配流感病毒中选择具有抗原蛋白质(x)的流感病毒(X)。