(EN) The present inventors provide methods for modifying the isoelectric point of an antibody while retaining its antigen-binding activity, comprising modifying the charge of at least one exposable amino acid residue on the surface of the complementarity determining region (CDR). The present invention also provides methods for purifying multispecific antibodies, comprising modifying isoelectric point, and methods for improving the plasma pharmacokinetics of antibodies, comprising modifying isoelectric point. The present invention further provides antibodies with a modified isoelectric point, pharmaceutical compositions comprising the antibodies as an active ingredient, and methods for producing the antibodies and compositions.
(ZH) 本发明人等提供：通过改变能够暴露于互补性决定区(CDR)表面的至少1个氨基酸残基的电荷，在保持抗体的抗原结合活性的同时改变抗体的等电点的方法。本发明还提供通过改变等电点来纯化多特异性抗体的方法和通过改变等电点来改善抗体的血浆中药物动力学的方法。并且，本发明提供等电点被改变的抗体、含有该抗体作为有效成分的医药组合物及其制造方法。