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Paramétrages

Paramétrages

1. US20130345402 - Devices and Methods for Integrated Continuous Manufacturing of Biological Molecules

Office États-Unis d'Amérique
Numéro de la demande 13795346
Date de la demande 12.03.2013
Numéro de publication 20130345402
Date de publication 26.12.2013
Type de publication A1
CIB
C CHIMIE; MÉTALLURGIE
07
CHIMIE ORGANIQUE
K
PEPTIDES
1
Procédés généraux de préparation de peptides
14
Extraction; Séparation; Purification
34
par filtration, ultrafiltration ou osmose inverse
CPC
Déposants Bayer Healthcare LLC
Inventeurs Vogel Jens Holger
Giovannini Roberto
Konstantinov Konstantin B.
Nguyen Huong My
Wu Paul
Titre
(EN) Devices and Methods for Integrated Continuous Manufacturing of Biological Molecules
Abrégé
(EN)

The present invention relates to a process and apparatus for purifying a molecule of interest from a heterogeneous clarified fluid mixture. The apparatus of the invention generally comprises a continuous perfusion fermentation system, a continuous particle removal system integrated with the perfusion fermentation system; and a continuous purification system integrated with the particle removal system, which is maintained under sterile conditions. The process comprises filtering a heterogeneous clarified fluid mixture by continuous ultrafiltration at a specific flow rate below the transition point of the molecule of interest in the pressure-dependent region of the flux versus TMP curve, wherein the specific flow rate is maintained substantially constant throughout the continuous ultrafiltration.