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1. WO2020109504 - UTILISATION D'OLIGODENDROCYTES À PARTIR DE CELLULES SOUCHES NEUROECTODERMIQUES ORALES DANS LA RÉPARATION DU SYSTÈME NERVEUX

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

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CLAIMS

1 ) A method for inducing differentiation of oral neuroectodermal stem cells into oligodendrocytes, said method comprising:

a) a step of differentiating said neuroectodermal oral stem cells into neuroepithelial stem cells in a neurogenic culture medium comprising DMEMf12, N 1 supplement, FGFb and EGF.

b) a step of differentiating the neuroepithelial stem cells obtained in step a) into neural stem cells in a neurogenic culture medium comprising DMEMf12, N1 supplement, Noggin protein, FGFb, retinoic acid and SHH factor.

c) a step of differentiating the neural stem cells obtained in step b) into oligodendrocyte pre-precursor cells in a neurogenic culture medium comprising DMEMf12, N1 supplement, B27 supplement and FGFb.

d) a step of differentiating the oligodendrocyte pre-precursor cells obtained in step c) into oligodendrocyte precursor cells in a neurogenic culture medium comprising

DMEMf12, PDGF-AA and SHH factor.

e) a step of differentiating oligodendrocyte precursor cells obtained in step d) into oligodendrocytes, and maturing said oligodendrocytes, in a neurogenic culture medium comprising DMEMf12, IGF-1 , biotin, CAMP, PDGF-AA, L-glutamine or GlutamaxTM and NT3.

2) A method according to claim 1 , wherein the neuroectodemic oral stem cells are derived from gingival tissue.

3) A cell population comprising oligodendrocytes obtained by the method according to claim 1 or 2, said oligodendrocytes expressing the Msx1 gene.

4) Cell population as defined in claim 3, for use in cell therapy.

5) Cell population for use according to claim 4, for the treatment of head injuries.

6) Culture medium for the differentiation of oral neuroectodermal stem cells into neuroepithelial stem cells comprising DMEMf12, N 1 supplement, FGFb and EGF.

7) Culture medium for the differentiation of neuroepithelial stem cells into neural stem cells comprising DMEMf12, N1 supplement, Noggin protein, FGFb, retinoic acid and SHH factor.

8) Culture medium for the differentiation of neural stem cells into oligodendrocyte pre-precursor cells comprising DMEMf12, N1 supplement, B27 supplement and FGFb.

9) Culture medium for the differentiation of oligodendrocyte pre-precursor cells into oligodendrocyte precursor cells comprising DMEMf12, PDGF-AA and SHH factor.

10) Culture medium for the differentiation of oligodendrocyte precursor cells into oligodendrocytes, and for the maturation of said oligodendrocytes, comprising DMEMf12, IGF-1 , biotin, CAMP, PDGF-AA, L-glutamine or GlutamaxTM and NT3.