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1. WO2019048690 - ENRICHISSEMENT DE CELLULES COEXPRIMANT NKX6.1 ET LE PEPTIDE C, DÉRIVÉES IN VITRO À PARTIR DE CELLULES SOUCHES

Numéro de publication WO/2019/048690
Date de publication 14.03.2019
N° de la demande internationale PCT/EP2018/074390
Date du dépôt international 11.09.2018
CIB
C12N 5/071 2010.01
CCHIMIE; MÉTALLURGIE
12BIOCHIMIE; BIÈRE; SPIRITUEUX; VIN; VINAIGRE; MICROBIOLOGIE; ENZYMOLOGIE; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE
NMICRO-ORGANISMES OU ENZYMES; COMPOSITIONS LES CONTENANT; CULTURE OU CONSERVATION DE MICRO-ORGANISMES; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE; MILIEUX DE CULTURE
5Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
07Cellules animales ou tissus animaux
071Cellules ou tissus de vertébrés, p.ex. cellules humaines ou tissus humains
CPC
A01N 1/0284
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
1Preservation of bodies of humans or animals, or parts thereof
02Preservation of living parts
0278Physical preservation processes
0284Temperature processes, i.e. using a designated change in temperature over time
A61K 35/39
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
37Digestive system
39Pancreas; Islets of Langerhans
C12N 2506/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2506Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
02from embryonic cells
C12N 2509/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2509Methods for the dissociation of cells, e.g. specific use of enzymes
C12N 2523/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2523Culture process characterised by temperature
C12N 5/0676
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0676Pancreatic cells
Déposants
  • NOVO NORDISK A/S [DK]/[DK]
Inventeurs
  • KIRKEGAARD, Jeannette, Schlichting
Données relatives à la priorité
17190412.111.09.2017EP
Langue de publication anglais (EN)
Langue de dépôt anglais (EN)
États désignés
Titre
(EN) ENRICHMENT OF NKX6.1 AND C-PEPTIDE CO-EXPRESSING CELLS DERIVED IN VITRO FROM STEM CELLS
(FR) ENRICHISSEMENT DE CELLULES COEXPRIMANT NKX6.1 ET LE PEPTIDE C, DÉRIVÉES IN VITRO À PARTIR DE CELLULES SOUCHES
Abrégé
(EN)
The present invention relates to method of enriching NKX6.1 and C-peptide co-expressing cell aggregates derived in vitro from stem cells said method comprising the steps of dissociating the endocrine cell aggregates into single cells, treating the single cells with cryopreservation medium and lowering temperature to obtain cryopreserved cells, thawing the cryopreserved cells; and re-aggregating the cells obtained after thawing into endocrine cells.
(FR)
La présente invention concerne un procédé d'enrichissement d'agrégats cellulaires coexprimant NKX6.1 et le peptide C, dérivés in vitro à partir de cellules souches, ledit procédé comprenant les étapes consistant à dissocier les agrégats de cellules endocrines en cellules individuelles, à traiter les cellules individuelles par un milieu de cryoconservation et à abaisser la température pour obtenir des cellules cryoconservées, à décongeler les cellules cryoconservées et à ré-agréger les cellules obtenues après décongélation en cellules endocrines.
Également publié en tant que
JP2020512652
MXMX/a/2020/002421
RU2020111055
SA520411466
Dernières données bibliographiques dont dispose le Bureau international