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1. (WO2019005955) TRANSCRIPTASE INVERSE AMÉLIORÉE ET PROCÉDÉS D'UTILISATION ASSOCIÉS
Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique


CLAIMS What A reverse transcriptase comprising a of The composition of comprises or more imitations in wherein the comprises the amino acid sequence set forth in SEQ The composition of 2 the transcriptase comprises least selected from the group consisting of and R353X relative to ID wherein denotes any amino The of wherein comprises at least one the group of and R353A relative to SEQ ID The composition of claim wherein the transcriptase comprises amino acid sequenc the group consisting of SEQ SEQ ΪΌ SEQ and SEQ ID The eompositi of claim the transcriptase comprises at least mutation selected the group consisting of mutation of terminal D binding mutation of the a to produce increased pairs within rigid sections of the tertiary of domain to of the d miiiatio of the catalytic a mutation wherein one or more residues or a domain is replaced with one more residues domain from enzyme of an anism other than 1 11 The composition of claim t of the DMA binding domain comprises at least one selected from the group consisting and X denotes any ami no wherein denotes of the residues t position 387 to position 427 of SEQ ID The composition of c w selected torn the group consisting Alanine and The composition of claim wherein the mutation the is selected from the group consisting o mutations portio of the and substitution of with a another reverse 1 The composition of wherein the produce increased pairs within rigid sections of the tertiary structure comprises at least selected the group consisting 1 and The composition of claim wherein X is Glutamic acid 1 The composition of claim w herein the mutation of the thumb comprises at least one selected from group consisting and wherein X denotes any no The o claim of the thumb domain comprises at least one selected from the group consisting The composition of claim wherein mutation of the catalytic site comprises at one selected from the group consisting A225 and wherein is any The wherein the mutation wherein a with domain a of organism other than c ri n is selected group consisting replacement of the finger domain of Ex matiirase of reverse and of domain of matiirase a domain of another matorase re ver se The composition of wherein mutation wherein or residues of Ex rase is with one or more residues derived of an comprises at least on selected from the group consisting and where X denotes amino The composition of claim ί the substitution mutation at least selected from the group consisting of 0 The composition of claim further comprising an that binding of primer to the of Ex The composition of claim th agent comprises a op The composition of claim wherein the agent comprises a nucleic acid mol cule derived The claim me agent comprises or a variant The composition of claim wherein t reverse has ne or properties selected from the gronp consisting of enhanced reduced error reduced and improved 23 A composition an isolated nucleic acid molecule encoding th reverse transcriptase of any one of A method of performing comprising contacting an RNA molecule a comprising reverse transcriptase comprising maturase or a variant of Ex The of wherein maturase comprises an amino acid sequence having greater about to the acid sequence set forth The of claim wherein the Ex maturase or variant of Ex maturase is used in an optimized reaction wherein the optimized reaction buffer comprises ris at concentration of about IOraM to about KCi at concentration of about I OOmM to about MgC at a concentration of about to about S DTT at a concentration of to about and wherein the reaction buffet has a of about to The method of claim wherein the optimized reaction butler further comprises one or more stabilizing The method of claim wherein the Ex maturase or a variant of inatorase is contacted agent reduces of primers to the or variant of The method of claim wherein the comprises a RN A The of claim the agent a nucleic molecule derive group II The of agent or a A kit comprising a polypeptide or a of The kit of claim comprises an amino acid having greater than about homology to the amino acid sequence forth SEQ The kit of further comprising that reduces of primers to the or of The of claim wherein the a A The of claim the agent comprises a nucleic acid molecule derived a grou int The kit of claim the agent comprises or a variant The of claim further an optimized wherein the optimized reaction buffer comprises Tris at a concentration of to about at a concentratio of about to about at concentration of about DTT at a concentration of to l and wherein the reaction buffer has a pH of about 8 to insufficientOCRQuality