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1. (WO2018162352) MILIEUX DE CULTURE COMPRENANT DES DIPEPTIDES DE N-ACYL-X-GLUTAMINE
Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

Claims

1. Cell culture media comprising L-glutamine from a set of N-acylated dipeptides Acyl-X-Q and L-glutamine from a set of other glutamine-sources Qsource in a defined molar ratio R = n(Acyl-X-Q) / n(Qsource);

wherein X is defined as an L-amino acid;

wherein Q is defined as L-glutamine attached via an amide bond to L-amino acid X;

wherein Acyl is defined as a C1-C7-acyl moiety attached via an amide bond to the amino-terminus of L-amino acid X;

wherein R is defined to be in the range of 0,03 to 20;

wherein n(Acyl-X-Q) is the total amount of substance of L-glutamine contained in the set of N- acylated dipeptides Acyl-X-Q in the culture media; and

wherein n(Qsource) is the total amount of substance of L-glutamine contained in the set of other glutamine sources Qsource in the culture media,

wherein the constituents of the set of other L-glutamine sources (Qsource) are selected from the following: Free L-glutamine, dipeptides Y-Q, or mixtures thereof, wherein Y is defined as one of the 20 genetically encoded L-amino acids,

wherein Q is defined as L-glutamine attached via an amide bond to L-amino acid Y, wherein the amide bond connecting Y and Q in dipeptide Y-Q is a regular backbone amide bond involving the carboxy terminus of amino acid Y and the amino terminus of glutamine Q.

2. Cell culture media according to claim 1 , wherein the cell culture media are serum free.

3. Cell culture media according to any one of claims 1 to 2, wherein the cell culture media are chemically defined.

4. Cell culture media according to any one of claims 1 to 3, wherein the only constituent of the set of other L-glutamine sources Qsource is dipeptide Y-Q, wherein Y is Alanine.

5. Cell culture media according to any one of claims 1 to 3, wherein the only constituent of the set of other L-glutamine sources Qsource is free L-glutamine.

6. Cell culture media according to any one of claims 1 to 5, wherein the total concentration of N-acylated dipeptides Acyl-X-Q is in the range of from 1 mM to 8 mM.

7. Cell culture media according to any one of claims 1 to 6, wherein the molar ratio R =

n(Acyl-X-Q) / n(Qsource) is defined to be in the range of 0,04 to 15,67.

8. Cell culture media according to any one of claims 1 to 7, wherein the set of N-acylated dipeptides Acyl-X-Q is defined as follows: One or more of acetyl-A-Q, acetyl-G-Q, acetyl-N- Q.

9. Cell culture media according to any one of claims 1 to 8, wherein the set of N-acylated dipeptides Acyl-X-Q is defined as follows: One or more of acetyl-A-Q, acetyl-G-Q.

10. Cell culture media according to any one of claims 1 to 9, wherein the set of N-acylated dipeptides Acyl-X-Q is defined as follows: Acetyl-A-Q.

1 1. Cell culture media according to any one of claims 1 to 10, wherein the cell culture media are chemically defined;

wherein the only constituent of the set of other L-glutamine sources Qsource is dipeptide Y-Q, wherein Y is Alanine;

wherein the molar ratio R = n(Acyl-X-Q) / n(Qsource) is defined to be in the range of 0,04 to 15,67; wherein the set of N-acylated dipeptides Acyl-X-Q is defined as follows: Acetyl-A-Q; and wherein the concentration of acetyl-A-Q is in the range of from 1 mM to 8 mM.

12. Use of cell culture media according to any one of claims 1 to 1 1 for culturing cells.

13. Process for the manufacture of a cell culture product, comprising the step: Contacting cells with a cell culture medium according to any one of claims 1 to 1 1.

14. Process according to claim 13, wherein the cells contacted with the cell culture medium are CHO cells.