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1. (WO2017137779) DISPOSITIF DE MONTAGE D'ÉCHANTILLON, ET PROCÉDÉ POUR MICROSCOPIE EN DIRECT
Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

CLAIMS

1 . A device for mounting a living, shape-changing or moving specimen in a chamber for microscopic examination, the device comprising:

a first part for mounting the device in or on the chamber;

an inlet port for receiving a flow of a liquid medium; and

a second part attached to the first part, the second part being arranged to extend into the chamber in use and to form a substantially straight channel between the second part and one or more walls of the chamber, the channel being for constraining the specimen in use;

wherein a first conduit extends from the inlet port, through at least part of the second part, to an outlet hole in the second part, for conveying the liquid medium into the chamber via the outlet hole.

2. A device as claimed in claim 1 , wherein the first part includes the inlet port.

3. A device as claimed in claim 1 or claim 2, wherein the second part is arranged so as to define said channel between the second part and a corner of the chamber.

4. A device as claimed in claim 3, wherein the second part is in the form of a shaft.

5. A device as claimed in claim 4, wherein the shaft is cylindrical.

6. A device as claimed in claim 4 or claim 5, wherein the shaft is aligned close to or with a corner of the first part, said corner of the first part corresponding to said corner of the chamber.

7. A device as claimed in any preceding claim, wherein the second part is provided with spacing means arranged to space the second part from one or more walls of the chamber.

8, A device as claimed in claim 7, wherein the spacing means comprise one or more collars arranged around the second part.

9. A device as claimed in any preceding claim, wherein the second part is arranged such that the channel is substantially vertical.

10. A device as claimed in any preceding claim, wherein the outlet hole is positioned at or near the bottom of the second part.

1 1 . A device as claimed in any preceding claim, wherein the outlet hole faces away from said channel.

12. A device as claimed in any preceding claim, further comprising an outlet port for conveying a circulated flow of the liquid medium away from the device.

13. A device as claimed in claim 12, further comprising an outlet mouth for receiving the circulated flow of the liquid medium from the chamber;

wherein a second conduit extends from the outlet mouth to the outlet port.

14. A device as claimed in claim 13, wherein the first part includes the outlet port.

15. A device as claimed in claim 14, wherein the outlet mouth is disposed on the underside of the first part.

16. A device as claimed in any preceding claim, wherein the first part incorporates an opening through which part of the specimen can extend in use.

17. A device as claimed in claim 16, wherein the opening is in a corner of the first part.

18. A device as claimed in claim 17 when dependent on claim 3, wherein said corner of the first part corresponds to said corner of the chamber.

19. A device as claimed in any of claims 16 to 18, wherein the opening is funnel-shaped.

20. A device as claimed in any preceding claim, being of unitary construction.

21 . A device as claimed in any preceding claim, wherein the surface of the second part has a chemical coating.

22. A device as claimed in any preceding claim, wherein the surface of the second part has a biologically-active coating.

23. A device as claimed in any preceding claim, wherein the surface of the second part is textured or profiled to physically challenge the specimen in use.

24. A device as claimed in any preceding claim, wherein the second part comprises one or more electrodes.

25. A device as claimed in any preceding claim, wherein the second part comprises one or more optical fibres.

26. A device as claimed in any preceding claim, wherein the surface of the second part comprises a physical support structure, matrix or scaffold for the specimen to adhere to in use.

27. An assembly comprising a device as claimed in any preceding claim mounted in a chamber.

28. An assembly as claimed in claim 27, wherein the chamber comprises a glass cuvette.

29. An assembly as claimed in claim 27 or claim 28 when dependent on claim 3, wherein, at said corner of the chamber, the walls of the chamber meet at an angle of substantially 90°.

30. An assembly as claimed in any of claims 27 to 29, further comprising a cover arranged to close the chamber with the device mounted therein.

31 . A method of examining a living, shape-changing or moving specimen, the method comprising:

preparing an assembly as claimed in any of claims 27 to 30 with the specimen being put in said channel;

causing a liquid medium to flow into and through the chamber via the inlet port and the outlet hole; and

examining the specimen within the channel using a microscope.

32. A method as claimed in claim 31 when dependent on any of claims 12 to 15, further comprising conveying the circulated flow of the liquid medium away from the device via the outlet port.

33. A method as claimed in claim 31 or claim 32, wherein the microscope employs Light Sheet Microscopy / Single Plane Illumination Microscopy.

34. A method as claimed in any of claims 31 to 33, further comprising automatically tracking and imaging the specimen as it changes shape or moves.

35. A method as claimed in any of claims 31 to 34, wherein the specimen comprises a living organism.

36. A method as claimed in claim 35, wherein the specimen comprises a plant or part thereof.

37. A method as claimed in claim 35, wherein the specimen comprises a fungus.

38. A method as claimed in claim 35, wherein the specimen comprises an animal such as a worm.

39. A method as claimed in any of claims 31 to 34, wherein the specimen comprises a growing, moving or shape-changing substance.

40. A method as claimed in claim 39, wherein the specimen comprises a growing crystal.

41 . A method as claimed in claim 39, wherein the specimen comprises a fluid and the method is for studying Brownian motion in the fluid.

42. A device substantially as herein described with reference to and as illustrated in any combination of the accompanying drawings.

43. An assembly substantially as herein described with reference to and as illustrated in any combination of the accompanying drawings.

44. A method of examining a living specimen substantially as herein described with reference to and as illustrated in any combination of the accompanying drawings.