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1. (WO2017001642) DISPOSITIF CAPTEUR
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CLAIMS

A biosensor device (1) for sensing an analyte, the device (1) comprising at least a sample inlet (10) for receiving a sample, affinity probes (111) selected so as to have an preferential binding to the analyte, a transducer (11) sensitive to a characteristic of the analyte and/or a label attached to the analyte and adapted to convert an interaction of the analyte with the affinity probes (111) into a readout signal, the transducer not being a Field Effect Transistor transducer, and a desalting unit (13) for desalting the received sample so as to reduce the response time and/or increase the signal of the transducer (11).

The device according to claim 1, wherein the desalting unit furthermore comprises a port (130) for receiving buffer fluid for being flown to the received sample.

The device (1) according to any of the previous claims, wherein the desalting unit (13) comprises a buffer fluid reservoir (131) for containing buffer fluid.

The device (1) according to any one of claims 2 or 3, wherein the desalting unit (13) comprises a mixer (132) for mixing received sample with the buffer fluid.

The device (1) according to any one of the preceding claims, wherein the transducer

(11) is an optical transducer.

The device (1) according to any one of the preceding claims, wherein the desalting unit (13) is integrated on or in a same substrate or in a same enclosure as the transducer (11).

The device (1) according to claim 6, wherein the desalting unit (13) comprises the port (130), the buffer fluid reservoir (131) and the mixer (132).

The device (1) according to any one of the preceding claims, wherein the device is an affinity-based sensing device having affinity probes on the transducer.

A diagnostic device comprising

a biosensor device (1) according to any of the previous claims for sensing an analyte and generating a sensing signal, and

an output unit for providing an output of said biosensor device, which can be used, alone or in combination with other factors, for basing a diagnosis on.

A diagnostic device according to claim 9, wherein the output device is adapted for outputting a signal representative for presence / absence or concentration of the analyte.

11. - A method for measuring the concentration of an analyte in a biological sample, the method comprising:

i. receiving a biological sample,

ii. desalting the sample, thereby obtaining a desalted sample, iii. measuring at least one signal of the desalted sample by means of an affinity-based sensing device based on affinity probes and a transducer, the transducer not being a Field Effect Transistor transducer,

iv. determining the concentration of the analyte in the sample using the at least one signal.

12. - The method according to claim 11, wherein the step ii. of desalting the sample comprises a step of bringing the sample to an ionic strength ranging from 10 nM to 150 mM, preferably from 10 mM to 150 mM.

13. - The method according to any of claims 11 or 12, wherein the step ii. of desalting the sample and the step iii. for measuring the at least one signal of the desalted sample are performed simultaneously or successively.

14. - The method according to any of claims 11 to 13, wherein the method furthermore comprises a step of comparing the at least one signal to a reference signal obtained with a standard solution.

15. - The method according to any of claims 11 to 14, wherein the step iii. of measuring the at least one signal of the desalted sample is performed over time, thus obtaining a measurement curve, and comprises a step of determining a slope of the measurement curve.

16. - The method according to any of claims 11 to 15, wherein the step ii of desalting the sample comprises a step of diluting the sample.

17. - The method according to any of claims 11 to 15, wherein the step ii. of desalting the sample comprises a step of performing electrodialysis.