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1. (WO2015173206) DERME ARTIFICIEL, PEAU ARTIFICIELLE, LEURS PROCÉDÉS DE PRÉPARATION, ET LEURS UTILISATIONS
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CLAIMS

1 . An artificial dermis comprising a first gel layer of clotted blood plasma comprising platelets and dermal cells, and a second gel layer of clotted blood plasma comprising platelets and dermal cells, and a polymer net situated between the first and the second gel layers.

2. An artificial dermis according to claim 1 wherein the polymer net comprises pores having an average diameter of between 10 μηι and 1000 μηι.

3. An artificial dermis according to claim 2, wherein the polymer net is made from interwoven polymer strands each strand having an average diameter of 10 μηι to 1000 μπι.

4. An artificial dermis according to any one of claims 1 to 3, wherein said polymer is biocompatible and biodegradable.

5. An artificial dermis according to claim 4 wherein said polymer is selected from the group consisting of poly(methyl methacrylate), polylactide, polyglycolide, poly(lactic- co-glycolic acid), polycaprolacton, polydioxanone, poly(glycolide-co-trimethylene- carbonate), polyglyconate and their mixtures.

6. An artificial dermis according to claim 5, wherein said polymer is poly(lactic-co- glycolic acid).

7. An artificial dermis according to any one of previous claims, wherein the dermal cells of the first and the second gel layer can be of the same or of different kind.

8. A method for the preparation of an artificial dermis according to any one of the previous claims, comprising the steps of:

(i) Providing a first reaction solution and optionally a second reaction solution both comprising blood plasma with platelets and dermal cells,

(ii) Clotting at least a portion of the first reaction solution to render a first gel layer of clotted blood plasma comprising platelets and dermal cells,

(iii) Applying a polymer net onto said first gel layer,

(iv) Clotting a further portion of the first reaction solution or at least a portion of a second reaction solution comprising blood plasma with platelets and dermal cells, onto the surface of the said polymer net to render a second gel layer of clotted blood plasma comprising platelets and dermal cells.

9. An artificial skin comprising an artificial dermis according to any one of claims 1 to 7, and at least a layer of epithelial cells.

10. An artificial skin according to the previous claim, wherein the at least one layer of epithelial cells is placed on the outside surface of the artificial dermis.

1 1 . A method for preparing an artificial skin according to claims 9-10, which comprises:

(iv) preparing an artificial dermis according to claims 1 to 7,

(v) seeding epithelial cells on at least one gel layer or polymer web; and

(vi) cultivating the cells until a layer of epithelial cells is produced.

12. Use of an artificial dermis or artificial skin according to the previous claims 1 to 7 or 9-10, for the preparation of a medicament.

13. Use of the artificial dermis or artificial skin according to claim 12, for the preparation of a medicament for the treatment of re-epithelialization of a patient in need thereof.

14. Use of the artificial dermis or artificial skin according to claim 13, wherein the re- epithelialization is of a mucosal, dermal or epidermal tissue.