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1. WO2012046149 - PROCÉDÉ D'AUGMENTATION DE LA PERMÉABILITÉ D'UNE BARRIÈRE ÉPITHÉLIALE

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

[ EN ]

WHAT IS CLAIMED IS:

1. A method for increasing the permeability of a cellular layer, the method comprising contacting the cellular layer with a surface of a device, the cellular layer comprising epithelial cells, the surface comprising a plurality of nanostructures formed on the surface, the nanostructures being arranged in a predetermined pattern, wherein subsequent to contact between the cellular layer and the surface, the cellular layer exhibits increased permeability to a drug compound.

2. The method according to claim 1, wherein subsequent to contact between the cellular layer and the surface, the transepithelial electrical resistance of the cellular layer is less than about 95% of the transepithelial electrical resistance of the layer prior to contact with the surface of the device.

3. The method according to claim 1, wherein subsequent to the contact between the cellular layer and the surface, the transepithelial electrical resistance of the cellular layer is less than about 85%, of the transepithelial electrical resistance of the layer prior to contact with the surface of the device.

4. The method according to claim 1, wherein subsequent to the contact between the cellular layer and the surface, the transepithelial electrical resistance of the cellular layer is less than about 70% of the transepithelial electrical resistance of the layer prior to contact with the surface of the device.

5. The method according to any of the preceding claims, wherein the pattern further includes microstructures, wherein the nanostructures have a cross-sectional dimension smaller than the microstructures.

6. The medical device of claim 5, further comprising second nanostructures having a cross-sectional dimension less than the cross-sectional dimension of the microstructures and greater than the cross-sectional dimension of the first nanostructures.

7. The method according to any of the preceding claims, wherein the cellular layer is skin.

8. The method according to any of the preceding claims, the method changing the structure of an intercellular junction.

9. The method according to claim 8, wherein the intercellular junction is a tight junction.

10. The method according to any of the preceding claims, wherein the surface is the surface of a microneedle.

11. The method according to any of the preceding claims, further comprising delivering the drug compound across the cellular layer.

12. The method according to claim 11, wherein the drug compound is a protein therapeutic.

13. The method according to claim 12, wherein the drug compound has a molecular weight greater than about 100 kDa.

14. The method according any of the preceding claims, wherein at least a portion of the nanostructures have a cross-sectional dimension of less than about 500 nanometers, and greater than about 5 nanometers.

15. The method according to any of the preceding claims, wherein the drug compound permeates across the cellular layer via paracellular transport.

16. The method according to any of the preceding claims, wherein the drug compound permeates across the cellular layer via transcellular transport.

17. The method according to any of the preceding claims, wherein the device includes a reservoir for holding the drug compound.