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1. WO2009073844 - PROMOTEURS DE PROTÉINES CHIMÈRES ET RICHES EN PROLINE POUR L'EXPRESSION DANS DES PLANTES

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

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What is claimed is:
1. A first polynucleotide molecule comprising a viral promoter enhancer molecule operably linked 5' to a plant proline rich protein promoter molecule, wherein the first polynucleotide molecule is capable of providing transcription of a linked second polynucleotide molecule different from a plant proline rich protein promoter without the viral promoter enhancer molecule.

2. The first polynucleotide molecule of claim 1 comprising a viral promoter enhancer molecule isolated from a DNA plant virus.

3. The first polynucleotide molecule of claim 1 comprising a plant proline rich protein promoter molecule selected from plant species from the group consisting of Medicago, Nicotiana, Arabidopsis, Oryza, Pisum, Zea, Saccharum, and Glycine species.

4. The first polynucleotide molecule of claim 1, comprising a polynucleic acid sequence selected from the group consisting of: (a) a sequence that exhibits at least about 85 percent identity to SEQ ID NO: 19, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO:27; (b) a sequence that exhibits at least about 90 percent identity to SEQ ID NO: 19, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO:27; (c) a fragment of SEQ ID NO: 19, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO:27, wherein the fragment comprises promoter activity; and (d) a sequence comprising SEQ ID NO: 19, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO:27.

5. The first polynucleotide molecule of claim 1, wherein the first polynucleotide molecule exhibits improved transcription in a plant cell of the second polynucleotide molecule

6. The first polynucleotide molecule of claim 2, wherein the viral promoter enhancer molecule is derived from a DNA plant virus selected from the group consisting of a Caulimovirus and a Badnavirus.

7. The first polynucleotide molecule of claim 6, wherein the Caulimo virus is a figwort mosaic virus or cauliflower mosaic virus.

8. The first polynucleotide molecule of claim 7, wherein the viral promoter enhancer molecule is selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, a fragment of SEQ ID NO:1 comprising an enhancer domain, and a fragment of SEQ ID NO:2 comprising an enhancer domain.

9. The first polynucleotide molecule of claim 1, wherein the plant proline rich protein promoter molecule is selected from the group consisting of: (a) SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or SEQ ID NO: 18.; and (b) a fragment of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or SEQ ID NO: 18, wherein the fragment comprises promoter activity.

10. The first polynucleotide molecule of claim 1, wherein the second polynucleotide molecule is a molecule of agronomic importance.

11. The first polynucleotide molecule of claim 10, wherein the second polynucleotide molecule provides a trait selected from the group consisting of herbicide tolerance, insect control, modified yield, fungal disease resistance, virus resistance, nematode resistance, bacterial disease resistance, plant growth and development, starch production, modified oils production, high oil production, modified fatty acid content, high protein production, fruit ripening, enhanced animal and human nutrition, biopolymers, environmental stress resistance, pharmaceutical peptides and secretable peptides, improved processing traits, improved digestibility, enzyme production, flavor, nitrogen fixation, hybrid seed production, fiber production, and biofuel production.

12. A stably transformed transgenic plant cell comprising the first and the second polynucleotide molecules of claim 1.

13. The stably transformed transgenic plant cell of claim 12, wherein the plant cell is a monocotyledonous plant cell selected from the group of plant cells consisting of a wheat cell, a maize cell, a rye cell, a rice cell, a corn cell, an oat cell, a barley cell, a turfgrass cell, a sorghum cell, a millet cell, and a sugarcane cell.

14. A transgenic plant comprising the first and the second polynucleotide molecules of claim 1.

15. The transgenic plant of claim 14, wherein the plant is a monocotyledonous plant.

16. The transgenic plant of claim 14, wherein the plant is a dicotyledonous plant.

17. A seed of the transgenic plant of claim 14, wherein the seed comprises the first and second polynucleotide molecules of claim 1.

18. The stably transformed transgenic plant cell of claim 12, wherein the plant cell is a dicotyledonous plant cell selected from the group of plant cells consisting of a tobacco cell, a tomato cell, a potato cell, a soybean cell, a cotton cell, a canola cell, a sunflower cell, and an alfalfa cell.

19. A method of modifying the phenotype of a transgenic crop plant, the method comprising expressing in the plant an expression cassette comprising a (i) PRP promoter or (ii) a chimeric polynucleotide promoter molecule comprising in operable linkage 5' to 3' a viral enhancer segment operably linked to a plant Prp promoter segment, which is (iii) operably linked to a nucleotide sequence coding for one or more molecules of agronomic importance; thereby producing a phenotype in the transgenic crop plant that is different from the phenotype of a crop lacking the expression cassette.

20. The method of claim 19, wherein the phenotype is reduced pest infestation or reduced pathogen infection of the crop plant, wherein the pest or pathogen is selected from the group consisting of arthropods, nematodes, fungi, bacteria, and viruses.

21. The method of claim 20, wherein the arthropod is selected from the group consisting of Acarina, Coleoptera, Diptera, Hemiptera, Homoptera, Hymenoptera, Lepidoptera, Orthoptera, and Thysanoptera.

22. The method of claim 20 wherein the nematode is selected from the group consisting of Heteroderidae, Meloidogynidae, and Pratylenchidae.

23. The method of claim 20, wherein the fungus is selected from the group consisting of Uredinales, Ustilaginales, Erysiphales, Oomycetes, Basidiomycetes, Ascomycetes, and Fungi Imperfecti.

24. The method of claim 20, wherein the bacterium is selected from the group consisting of Pseudomonas, Xanthomonas, Erwinia, and Corynebacterium.

25. The method of claim 20, wherein the virus is selected from the group consisting of Potyviruses, Luteoviruses, Gemini viruses, ϋarviruses, Comoviruses, Caulimoviruses, Cucumoviruses, Fijiviruses, Alfamoviruses, Badnaviruses, Bromoviruses, Furoviruses, Tospoviruses, and Tobamo viruses.

26. A polynucleotide sequence comprising a promoter functional in a plant cell, the promoter comprising in operable linkage 5' to 3' a plant viral promoter enhancer segment and a plant proline rich protein promoter segment, wherein expression of a gene of interest from the promoter functional in a plant cell is increased in the plant cell compared to expression of the gene of interest from the plant proline rich protein segment in the absence of the plant viral promoter enhancer segment.

27. The polynucleotide sequence according to claim 26 wherein the promoter comprises the sequence selected from the group consisting of (a) a sequence that exhibits at least about 85 percent identity to SEQ ID NO: 19, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO:27; (b) a sequence that exhibits at least about 90 percent identity to SEQ ID NO: 19, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO:27; (c) a fragment of SEQ ID NO: 19, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO:27, wherein the fragment comprises promoter activity; and (d) a sequence comprising SEQ ID NO: 19, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, or SEQ

ID NO:27.

28. The polynucleotide sequence according to claim 27 wherein the promoter comprises the sequence as set forth in SEQ ID NO: 19 or SEQ ID NO:20, or a fragment thereof with promoter activity.

29. The polynucleotide sequence according to claim 26 wherein the plant cell is from a plant selected from the group consisting of sugar beet, wheat, maize, rye, rice, corn, oat, barley, turf grass, sorghum, millet, Arabidopsis, sugarcane, tobacco, tomato, potato, soybean, cotton, canola, sunflower, alfalfa, milo, cucurbit, pigeon pea, vegetable, fruit tree, berry, and forage grass.

30. The polynucleotide sequence according to claim 29 wherein the plant cell is a soybean plant cell.

31. The polynucleotide sequence according to claim 30, wherein the soybean plant cell is further selected from the group consisting of a root cell, a stem cell, a leaf cell, a flower cell, and a seed cell.

32. The polynucleotide sequence according to claim 31 wherein the root cell is further selected from the group consisting of a epidermis cell, a root hair cell, a root tip cell, a endoderm cell, a vascular cell, a stele cell, and a cortex cell.

33. A nucleotide sequence comprising a plant proline rich protein promoter sequence, wherein the nucleotide sequence comprises substantially the sequence selected from the group consisting of SEQ ID NO:5 and SEQ ID NO:6.

34. The nucleotide sequence as set forth in claim 33, wherein the promoter sequence is a part of a vector for use in transforming a cell, and wherein the cell is selected from the group consisting of a plant cell, a fungal cell, and a bacterial cell.

35. A plant cell comprising a heterologous promoter comprising a Mt.prp2 promoter segment, wherein the Mt.prp2 promoter segment is selected from the group consisting of (a) SEQ ID NO:5 or SEQ ID NO:6; (b) a fragment of SEQ ID NO:5 or SEQ ID NO:6, wherein the fragment comprises promoter activity; and (c) a sequence that exhibits at least about 85 percent identity to SEQ ID NO:5 or SEQ ID NO:6.

36. A transgenic plant produced from the plant cell of claim 35.

37. A seed comprising the cell of claim 35.

38. A seed produced from the transgenic plant of claim 36, wherein the seed comprises the promoter segment.

39. The transgenic plant of claim 36, wherein (a) the promoter is operably linked to a gene of interest, (b) expression of the gene of interest from the promoter confers a desirable phenotype upon the plant, and (c) the phenotype is selected from the group consisting of resistance to plant pest infestation, resistance to plant pathogen infection, increased yield of seed or other desirable plant product, increased tolerance to drought, increased nitrogen utilization efficiency, increased photosynthetic capacity, increased heat tolerance, increased vigor, increased standability, and increased cold tolerance.