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1. WO2007102892 - DISPOSITIF DE CONTRÔLE DE RÉACTIONS EN CHAÎNE DE LA POLYMÉRASE

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[ EN ]

WHAT IS CLAIMED IS:
1. A method of calibrating temperature comprising:
cycling temperatures of a set of wells, wherein each well of the set comprises a sample with a spectrally distinguishable species;
measuring a signal from the spectrally distinguishable species for each well at a temperature during a first temperature cycle; and
calibrating the temperatures for measuring the signal from each well during subsequent temperature cycles.
2. The method of claim 1 , wherein the temperature cycles range from about 6O0C to about 95°C.
3. The method of claim 1 , wherein the spectrally distinguishable species is selected from the group consisting of dyes, reporters, and reagents.
4. The method of claim 1 , wherein the spectrally distinguishable species is selected from the group consisting of FAM, SYBR Green, VIC, JOE, TAMRA, NED, CY-3, Texas Red, CY-5, and ROX.
5. The method of claim 1 , wherein the temperature from the spectrally distinguishable species for each well is different during the first temperature cycle.

6. The method of claim 1 , wherein the temperature from the spectrally distinguishable species for each well is the same during the first temperature cycle.

7. A method comprising:
measuring a signal from a spectrally distinguishable species present in a first well from a set of wells at a first temperature;
heating the set of wells to a second temperature; and
measuring a signal from a spectrally distinguishable species present in at least one additional well from the set of wells at the second temperature.

8. The method of claim 7, wherein the first temperature is greater than or equal to an annealing temperature of DNA.
9. The method of claim 7, wherein the first temperature is greater than or equal to about 60° C.
10. The method of claim 7, wherein less than about 30 seconds elapse between measurements.
11. The method of claim 7, wherein less than about 20 seconds elapse between measurements.
12. The method of claim 7, wherein the second temperature is held for a period of time.
13. The method of claim 12, wherein the period of time is less than about five seconds.
14. The method of claim 7, wherein the second temperature is not held for a period of time.
15. A method comprising:
measuring a signal from a spectrally distinguishable species present in at least one well at a temperature other than an annealing temperature during a first measurement period; and
measuring a signal from the same at least one well at the temperature during subsequent measurement periods.
16. The method of claim 15, wherein a first measurement period comprises heating the at least one well and cooling the at least one well.
17. A method of reducing the run time of a reaction, comprising:
cycling a thermal cycler block having a set of wells from about 6O0C to about 95°C in a linear relationship over a period of time; and measuring a signal from each well in the set of wells.
18. The method of claim 17, wherein the signal from each well is measured in less than about 30 seconds.
19. The method of claim 17, wherein the signal from each well is measured in less than about 20 seconds.
20. The method of claim 17, wherein each well from the set of wells comprises a spectrally distinguishable species.