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Paramétrages

Paramétrages

1. WO2005068494 - TOXINE BOTULINUM CHIMERE DE TYPE E

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

ALLE0014-105 PATENT

17355CIP5
What is claimed is:

1. A modified toxin comprising a modified light chain of a botulinum toxin type E, wherein the modified light chain comprises:
an amino acid sequence SEQ ID NO: 144 (PFVNKQFN ) at the N-terminal of the modified light chain; and
an amino acid sequence SEQ ID NO: 112 (xExxxLL) at the C-terminal of the modified light chain, wherein x is any amino acid.

2. The toxin of claim 1 wherein the amino acid sequence SEQ ID NO: 144 (PFVNKQFN) is part of the first 12 amino acids of the N-terminal of the modified light chain, and the amino acid sequence SEQ ID NO: 112 (xExxxLL) is part of the last 27 amino acids of the C-terminal of the modified light chain.

3. The toxin of claim 1 wherein the amino acid sequence SEQ ID NO: 144 (PFVNKQFN) is part of the first 8 amino acids of the N-terminal of the modified light chain, and the amino acid sequence xExxxLL (SEQ ID NO: 112) is part of the last 23 amino acids of the C-terminal of the modified light chain.

4. The toxin of claim 1 wherein:
the amino acid sequence SEQ ID NO: 144 (PFVNKQFN) is the first 8 amino acids of the N-terminal of the modified light chain; and
the amino acid sequence SEQ ID NO: 112 (xExxxLL) is prior to the last 16 amino acids at the C-terminal of the modified light chain.

5. The toxin of claims 1, 2, 3, or 4 wherein the toxin further comprises a heavy chain of a Clostridial toxin.

6. The toxin of claim 5 wherein the heavy chain is a heavy chain of a
botulinum toxin type A, B, Ci, D, E, F, or G.

ALLE0014-105 PATENT

17355CIP5
7. The toxin of claim 5 wherein the heavy chain is a heavy chain of a
botulinum toxin type E.

8. A modified toxin comprising an amino acid sequence of SEQ ID NO: 142.

9. The toxin of claim 8 further comprising a heavy chain of a Clostridial toxin.

10. The toxin of claim 9 wherein the heavy chain is a heavy chain of a botulinum toxin type A, B, C\, D, E, F, or G.

11. The toxin of claim 9 wherein the heavy chain is a heavy chain of a botulinum toxin type E.

12. A nucleic acid sequence comprising a region that encodes an amino acid sequence SEQ ID NO: 142.

13. The nucleic acid sequence of claim 12 comprising SEQ ID NO: 143.

14. The nucleic acid sequence of claim 12 further comprising a region that encodes a heavy chain of a Clostridial toxin.

15. The nucleic acid sequence of claim 14 wherein the heavy chain is a heavy chain of a botulinum toxin type A, B, Ci, D, E, F, or G.

16. The nucleic acid sequence of claim 14 wherein the heavy chain is a heavy chain of a botulinum toxin type E.