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1. (WO2005066342) CHAMBRE A CHAMP ELECTRIQUE NON UNIFORME POUR FUSION CELLULAIRE
Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

Claims
1. An apparatus for carrying out fusion of biological cells, comprising:
an inner electrode having a first electrode radius (rl) and an electrode height,
an outer electrode having a second electrode radius (r2) and said electrode height, wherein said inner
electrode and said outer electrode are concentric,
a gap between said inner electrode and said outer electrode, wherein the size of said gap is the difference between said second electrode radius and said first electrode radius, and wherein a cell fusion volume is defined by said electrode height, said gap, said first electrode radius (rl) , and said second electrode radius (r2) , wherein said first electrode radius, said second electrode radius, and said gap are selected in accordance with a predetermined range of selectable ratios (rl/r2) of said first electrode radius to said second electrode radius, wherein said range of selectable ratios is from 0.7 to 0.9, a selected gap limited by said range of selectable ratios, and a determined ratio of said
selectable ratios based on said selected gap, such that compression between the biological cells and permeability between cell membranes are maximized and temperature rise is minimized for providing cell fusion in said cell fusion volume .

2. The apparatus of claim 1 wherein said cell fusion volume has a volume of greater than 1 milliliter.

3. The apparatus of claim 1 wherein said ratio of said first electrode radius to said second electrode radius is in a range of 0.75 to 0.9.

The apparatus of claim 1 wherein:
said ratio of said first electrode radius to said second electrode radius is in a range of 0.8 to 0.85, and said gap is in a range of 2 to 10 millimeters.

5. The apparatus of claim wherein:
said ratio of said first electrode radius to said second electrode radius is 0.83 , and
said gap is 4 millimeters.

6. A method for selecting an inner electrode, an outer electrode, and a gap between the inner electrode and the outer electrode for a cell fusion chamber for fusing biological cells, comprising the steps of:
determining two of a first electrode radius of the inner electrode, a second electrode radius of the outer electrode, and the gap between the inner electrode and the outer electrode,
setting the ratio (rl/r2) of the first electrode radius to the second electrode radius to a value in a range between 0.7 to 0.9, and
calculating the third of the first electrode radius of the inner electrode, the second electrode radius of the outer electrode, and the gap between the inner electrode and the outer electrode, based on the set value of the ratio, such that compression between the biological cells and permeability between cell membranes are maximized and temperature rise is minimized for providing cell fusion in the cell fusion chamber.

7. The method of claim 6 wherein:
the ratio of the first electrode radius to the second electrode radius is set to a value in a range between 0.8 to 0.85, and
the gap is in a range of 2 to 10 millimeters.

8. An apparatus for carrying out fusion of biological cells, comprising: a non-conductive base member,
a conductive outer electrode supported on said base member, wherein said outer electrode includes a concave outer electrode surface which has an outer electrode radius (r2) and has an electrode height,
a conductive inner electrode supported on said base member, wherein said inner electrode includes a convex inner electrode surface which has an inner electrode radius (rl) and has the electrode height, wherein said outer electrode surface and said inner electrode surface are spaced apart from each other by a gap which defines a fusion chamber,
a non-conductive outer electrode cover member
supported by said outer electrode, and
a non-conductive inner electrode cover member
supported by said inner electrode, wherein said outer electrode cover member and said inner electrode cover member define an access channel, wherein said access channel is in communication with said fusion chamber.

9. The apparatus of claim 8 wherein:
said non-conductive outer electrode cover member includes a concave outer cover member surface which has an outer cover member radius,
said non-conductive inner electrode cover member includes a convex inner cover member surface which has an inner cover member radius, and
said outer cover member radius is equal to said outer electrode radius, and said inner cover member radius is equal to said inner electrode radius, whereby said access channel is in registration with said fusion chamber.

10. An apparatus for carrying out fusion of biological cells, comprising:
a non-conductive support member,
a conductive outer electrode supported in a
horizontal orientation by said support member, wherein said outer electrode includes a conductive concave outer electrode surface which has an outer electrode radius (r2) and has an electrode width,
a conductive inner electrode supported in a
horizontal orientation by said support member above said outer electrode, wherein said inner electrode includes a conductive convex inner electrode surface which has an inner electrode radius (rl) and has said electrode width, and
non-conductive vertically oriented end walls located at ends of said outer electrode and said inner electrode, wherein said outer electrode surface and said inner electrode surface are spaced apart from each other by a gap, and wherein said gap and said vertically oriented end walls define a fusion chamber.

11. The apparatus of claim 10 wherein:
said outer electrode includes a non-conductive outer electrode support portion which supports said conductive outer electrode surface, and
said inner electrode includes a non-conductive inner electrode support portion which supports said conductive inner electrode surface.

12. The apparatus of claim 10 wherein said non-conductive support member, said non-conductive outer electrode support portion, said non-conductive inner electrode support portion, and said non-conductive vertically oriented end walls are formed as an integrated molded unit.

13. The apparatus of claim 10, further including an input/output port supported by said support member, wherein said input/output port is in communication with said fusion chamber.

14. The apparatus of claim 10, further including a filter pressure relief valve supported by said support member, wherein said filter pressure relief valve is in
communication with said fusion chamber.