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1. WO2004101708 - MATERIAUX POLYMERIQUES FLUORESCENTS CONTENANT DES COLORANTS DE RHODAMINE LIPOSOLUBLES

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What is Claimed:

1. A fluorescent polymeric material comprising a polymer and at least one lipid-soluble rhodamine dye comprising one of the following core structures:

where R1 R2, R2' , R3', R3", R4, R4', R5', R5, R6", R6', R7', R7, R8, R9, R11 R12, R13, R14 and R15 are, independently, selected from hydrogen or the same or different substitaent groups having no more than 40 atoms,

where at least one of R3', R3", R6" and R6' is a lipophilic substitaent;

where R9 in core structure (II) is a nonaromatic substitaent;

where, with respect to core structures (I), (III) and (IV), if R 3" and R6" are each phenyl or substitated phenyl and R3' and R6' are each hydrogen then at least two of R11, R12, R13, R14 and R15 must be other than hydrogen; and

where, with respect to core structures (I), (III) and (IV), R1 and R8 are not, simultaneously, pendant or fused benzo, naptho or polycyclic aryleno groups.

2. A fluorescent polymeric material comprising a polymer activated with functional groups suitable for covalent attachment of molecules thereto, said polymer having incorporated internally therein at least one lipid-soluble rhodamine dye comprising one of the following core structures:

where R1 R2, R2', R3', R3", R4, R4', R5', R5, R6", R6', R7', R7, R8, R9, R11 R12, R13, R14 and R15 are, independently, selected from hydrogen or the same or different substituent groups having no more than 40 atoms,

where at least one of R3', R3", R6" and R6' is a lipophilic substitaent;

where R9 in core structure (II) is a nonaromatic substituent; and

where, with respect to core structures (I), (III) and (IV), R1 and R8 are not, simultaneously, pendant or fused benzo, naptho or polycyclic aryleno groups.

3. A fluorescent polymeric material comprising a polymeric particle that swells in organic solvent, said polymeric particle having incorporated internally therein at least one lipid-soluble rhodamine dye comprising one of the following core structures:

where R1 R2, R2', R3', R3", R4, R4', R5', R5, R6", R6', R7', R7, R8, R9, R11 R12, R13, R14 and R15 are, independently, selected from hydrogen or the same or different substituent groups having no more than 40 atoms,

where at least one of R3 , R3 , Rδ and R6 is a lipophilic substitaent;

where R9 in core structure (II) is a nonaromatic substitaent; and

where, with respect to core structures (I), (III) and (IV), R1 and R8 are not, simultaneously, pendant or fused benzo, naptho or polycyclic aryleno groups.

4. A fluorescent polymeric material comprising a polymeric particle formed from a crosslinked or uncrosslinked polymer selected from polystyrene homopolymer and

copolymers of styrene polymerized with one or more other monomers, said polymeric particle having incorporated internally therein at least one lipid-soluble rhodamine dye comprising one of the following core structures:

where R1 R2, R2', R3', R3", R4, R4', R5', R5, R6", R6', R7', R7, R8, R9, R11 R12, R13, R14 and R15 are, independently, selected from hydrogen or the same or different substitaent groups having no more than 40 atoms,

where at least one of R3', R3", R6" and R6' is a lipophilic substitaent;

where R9 in core structure (II) is a nonaromatic substitaent; and

where, with respect to core structures (I), (III) and (IV), R1 and R8 are not, simultaneously, pendant or fused benzo, naptho or polycyclic aryleno groups.

5. The fluorescent polymeric material of any one of Claims 1-4 wherein at least one of R3', R3", R6" and R6' is a lipophilic substituent selected from (C4-C20) alkyls and heteroalkyls, (C5-C20) aryls and heteroaryls, and (C6-C40) arylalkyls or heteroaryl alkyls.

6. The fluorescent polymeric material of any one of Claims 1-4 wherein at least one of R3', R3", R6" and R6' is a lipophilic substitaent is selected from (C7-C20) alkyls.

7. The fluorescent polymeric material of any one of Claims 1-4 wherein at least two of R3', R6", R3" and R6" are the same or different lipophilic substitaent.

8. The fluorescent polymeric material of any one of Claims 1-4 wherein all of R3', R6", R3" and R6" are the same or different lipophilic substitaent.

9. The fluorescent polymeric material of any one of Claims 1-4 wherein the substituents in core structures (I), (II), (III) and (IV) are further defined as follows:

(i.) with respect to core structure (I),

R1 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R1 is taken together with R2 form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R2 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R2 is taken together with R1 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substituted with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R2 is taken together with R3 to form a 5- or 6-membered ring which is optionally substituted with one or more of the same or different Ra or suitable Rb groups,

R3' is selected from hydrogen, (C4-C20) alkyl or heteroalkyl optionally substituted with one or more of the same or different R groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R3' is taken together with R2 to form a 5- or 6-membered ring which is optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R3 is selected from (C4-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R3 is taken together with R4 to form a 5- or 6-membered ring which is optionally substitated with one or more of the same or different Ra or suitable Rb groups, R4 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substituted with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively R4 is taken together with R3 to form a 5- or 6-membered ring which is optionally substitated with one or more of the same or different Ra or suitable Rb groups, R5 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substituted with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb group or, alternatively R5 is taken together with R6 to form a 5- or 6-membered ring which is optionally substitated with one or more of the same or different Ra or suitable Rb groups, R6 is selected from (C4-C20) alkyl or heteroalkyl optionally substituted with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substituted with one or more of the same or different Ra or suitable Rb groups or, alternatively R6 is taken together with R5 to form a 5- or 6-membered ring which is optionally substituted with one or more of the same or different Ra or suitable Rb groups, R6' is selected from hydrogen, (C4-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substituted with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R6' is taken together with R7 to form a 5- or 6-membered ring optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R7 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substituted with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substitated with one or more of the same or different Ra or Rb groups, or, alternatively, R7 is taken together with R6' to form a 5- or 6-membered ring optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R 7 is taken together with R8 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R8 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R8 together with R7 form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R11 and R15 are each, independently of one another, selected from hydrogen, halo, (C1-C20) alkyl, haloalkyl, -ORy, -SRy, -SORy, -SO2Ry, -C(O)ORy, -S(O)2ORy, amide, sulfonamide and nitrile,

R12, R13 and R14 are each, independently of one another, selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substituted with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups,

Rx is selected from -NRcRc, -ORd, -SRd, halo, haloalkyl, -CN, -NC, -OCN, -SCN, -NO, -NO2, -N3, -S(O)Rd, -S(O)2Rd, -S(O)2ORd, -S(O)NRcRc, -S(O)2NRcRc, -OS(O)Rd, -OS(O)2Rd, -OS(O)2ORd, -OS(O)2NRcRc, -C(O)Rd, -C(O)ORd, -C(O)NRcRc, -C(NH)NRcRc, -OC(O)Rd, -OC(O)ORd, -OC(O)NRcRc and -OC(NH)NRcRc,

Ry is selected from (C1-C20) alkyls or heteroalkyls optionally substituted with lipophilic substituents, (C5-C20) aryls or heteroaryls optionally substitated with lipophilic substitaents and (C6-C26) arylalkyl or heteroarylalkyls optionally substitated with lipophilic substitaents,

Ra is selected from hydrogen, (C1-C8) alkyl or heteroalkyl, (C5-C20) aryl or heteroaryl and (C6-C28) arylalkyl or heteroarylalkyl,

Rb is selected from -NRcRc, =O, -ORd, =S, -SRd, =NRd, =NORd, halo, haloalkyl, , -CN, -NC, -OCN, -SCN, -NO, -NO2, =N2, -N3, -S(O)Rd, -S(O)2Rd, -S(O)2ORd, -S(O)NRcRc, -S(O)2NRcRc, -OS(O)Rd, -OS(O)2Rd, -OS(O)2ORd, -OS(O)2NRcRc, -C(O)Rd, -C(O)ORd, -C(O)NRcRc, -C(NH)NRcRc, -OC(O)Rd, -OC(O)ORd, -OC(O)NRcRc and -OC(NH)NRcRc,

each Rc is independently hydrogen or Rd , or, alternatively, each Rc is taken together with the nitrogen atom to which it is bonded to form a 5 to 8-membered saturated or unsaturated ring which may optionally include one or more of the same or different additional heteroatoms and which may optionally be substituted with one or more of the same or different Ra or Rd groups,

each Rd is independently Ra or Ra substitated with one or more of the same or different Ra or Re groups, and

each Re is selected from -NRaRa, =O, -ORa, =S, -SRa, =NRa, =NORa, halo, haloalkyl, -CN, -NC, -OCN, -SCN, -NO, -NO2, =N2, -N3, -S(O)Ra, -S(O)2Ra, -S(O)2ORa, -S(O)NRaRa, -S(O)2NRaRa, -OS(O)Ra, -OS(O)2Ra, -OS(O)2ORa, -OS(O)2NRaRa, -C(O)Ra, -C(O)ORa, -C(O)NRaRa, -C(NH)NRaRa, -OC(O)Ra, -OC(O)ORa, -OC(O)NRaRa and -OC(NH)NRaRa; and

(ii.) with respect to core structure (II),

R1, R2, R3', R3", R4, R5, R6", R6', R7, R8, Rx, Ra, Rb, Rc, Rd, and Re are as first defined in section 5.6 with respect to core structure (I), and

R9 is selected from hydrogen alkyl, halo, haloalkyl, or nitrile;

(iii.) with respect to core structure (III),

R1, R3", R5, R6", R6', R7, R8, R11, R12, R13, R14, R15 Rx, Ry, Ra, Rb, Rc, Rd, and Re are as first defined in section 5.6 with respect to core structure (I),

R2 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substituted with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substituted with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substituted with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R2 is taken together with R1 or R2 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable R groups,

R2 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substituted with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substituted with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R 2 ' is taken together with R2 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R2 is taken together with R3' to form a 5- or 6-membered ring which is optionally substitated with one or more of the same or different Ra or suitable R groups,

R3 is selected from hydrogen, (C4-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substituted with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R3 is taken together with R2 to form a 5- or 6-membered ring which is optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R4 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substituted with one or more of the same or different Ra or suitable Rb groups, or, alternatively R4 is taken together with R3" to form a 5- or 6-membered ring which is optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R4 is taken together with R4 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups, and

R4 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substituted with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substituted with one or more of the same or different Ra or suitable Rb groups, or, alternatively R4 is taken together R4 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substituted with one or more of the same or different Ra or suitable Rb groups; and

(iv.) with respect to core structure (IV),

R1, R3", R6", R8, R11, R12, R13, R14, R15 Rx, Ry, Ra, Rb, Rc, Rd, and Re are as first defined in section 5.6 with respect to core structure (I),

R2, R2', R3', R4, and R4' are as defined in section 5.8 with respect to core structure (III),

R5' is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substituted with one or more of the same or different Ra or suitable Rb group or, alternatively R5 is taken together with R5 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substituted with one or more of the same or different Ra or suitable Rb groups,

R5 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substituted with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb group or, alternatively R5 is taken together with R6" to form a 5- or 6-membered ring which is optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R5 is taken together with R5' to form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R6' is selected from hydrogen, (C4-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R6 is taken together with R7 to form a 5- or 6-membered ring optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R7 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substituted with one or more of the same or different Ra or Rb groups, or, alternatively, R7 is taken together with R6 to form a 5- or 6-membered ring optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R7 is taken together with R7 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups, and

R7 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different R groups, (C5-C20) aryl or heteroaryl optionally substituted with one or more of the same or different Ra or suitable Rb. groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substituted with one or more of the same or different Ra or Rb groups, or, alternatively, R7 is taken together with R7 or R8 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups.

10. The fluorescent polymeric material of Claim 9 wherein the lipid soluble rhodamine dye comprises core structure (I).

11. The fluorescent polymeric material of Claim 9 wherein the lipid soluble rhodamine dye comprises core structure (I) and, additionally, the substitaents therein comprise one or more of the following featares:

R1 and R8 are each, independently of one another, selected from hydrogen, (C1-C8) alkyl, halo, and -CF3, or are part of a fused aryl or heteroaryl group with R2 and R7, respectively;

R2 and R7 are each, independently of one another, selected from hydrogen, (C1-C8) alkyl, halo, -CF3, -CN, carboxylate, ester, sulfone, amino, amido and oxoether, or are part of a fused aryl or heteroaryl group with R1 and R8, respectively, or are part of a 5 or 6 membered ring with R3 and R6 , respectively;

R3 and R6 are each, independently of one another, selected from hydrogen and (C4-C20) alkyl, or are part of a 5 or 6 membered ring with R2 and R7, respectively;

R3" and R6 are each, independently of one another, selected from hydrogen and (C4-C20) alkyl or are part of a 5 or 6 membered ring with R4 and R5, respectively;

at least two of R3', R3", R6', R6" are not hydrogen;

R4 and R5 are each, independently of one another, selected from hydrogen, (C1-C8) alkyl, halo, -CF3, aryl, heteroaryl, -CN, carboxylate, ester, and sulfone, or are part of a 5 or 6 membered ring with R3" and R6' , respectively;

R11 and R15 are each, independently, selected from hydrogen, halo, ester, amide, sulfonamide, (C 1 -C20) alkyl, -ORe, -SRe, -CN and sulfone; and

R12, R13 and R14 are each, independently of one another, selected from hydrogen, halo, alkyl, aryl, (C4 to C20) alkyl ester, sulfone, -CF3 and -CN.

12. The fluorescent polymeric material of Claim 9 wherein the lipid soluble rhodamine dye comprises core structure (I) and, additionally, the substituents therein comprise one or more of the following featares:

R1 and R8 are each, independently of one another, selected from hydrogen, (C1-C8) alkyl, and halo, or are part of a fused aryl or heteroaryl group with R2 and Rs, respectively;

R2 and R7 are each, independently of one another, selected from hydrogen, (C1-C8) alkyl, and halo, or are part of a fused aryl or heteroaryl group with R1 and R8 respectively, or are part of a 5 or 6 membered ring with R3 and R6 , respectively;

R3' and R6' are each the same or different (C4-C20) alkyl;

R3 and R6 are each, independently of one another, selected from hydrogen and the same or different (C4-C20) alkyl, or are part of a 5 or 6 membered ring with R4 and R5, respectively;

R4 and R5 are each, independently of one another, selected from hydrogen, (C1-C8) alkyl, aryl and heteroaryl and halo, or are part of a 5 or 6 membered ring with R3 and R6 , respectively;

R11 and R15, independently of one another, selected from hydrogen, fluoro, chloro, (C 1 -C20) alkyl, -ORe and -SRe; and

R12, R13 and R14 are each, independently of one another, selected from hydrogen and halo.

13. The fluorescent polymeric material of Claim 9 wherein the lipid soluble rhodamine dye comprises one of the following structural embodiments of core structure (I):











where each Rz is independently selected from hydrogen, Rb, (C1-C20) alkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb group; and

R1, R2, R3', R3", R4, R5, R6', R6", R7, R8, Rn, R12, R13, R14, R15, Ra and Rb are as previously defined.

14. The fluorescent polymeric material of Claim 9 wherein the lipid soluble rhodamine dye is one of the following embodiments of core structure (I):











15. The fluorescent polymeric material of Claim 9 wherein the lipid soluble rhodamine dye comprises core structure (II).

16. The fluorescent polymeric material of Claim 9 wherein the lipid soluble rhodamine dye comprises core structure (II) and, additionally, the substituents therein comprise one or more of the following featares:

R1 when taken together with R2 forms part of a fused benzo, naptho or polycyclic aryleno group that is optionally substitated with one or more of the same or different Ra or suitable Rb groups;

R7 when taken together with R8 forms part of a fused benzo, naptho or polycyclic aryleno group that is optionally substitated with one or more of the same or different Ra or suitable Rb groups; and

R9 is hydrogen.

17. The fluorescent polymeric material of Claim 9 wherein the lipid soluble rhodamine dye comprises one of the following structural embodiments of core structure (II):





wherein each RQ is independently selected from hydrogen, (C1-C20) alkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb group; and Ra and Rb are as previously defined.

18. The fluorescent polymeric material of Claim 9 wherein the lipid soluble rhodamine dye is selected from the following embodiments of core structure (II):





19. The fluorescent polymeric material of Claim 9 wherein the lipid soluble rhodamine dye comprises core structure (III).

20. The fluorescent polymeric material of claim 9 wherein the lipid soluble rhodamine dye comprises core structure (III) and further comprises one or more substitated or unsubstitated five or six membered fused rings that comprise, as part of the ring, a nitrogen atom from either the exocyclic amine or exocyclic imminium group and one of R2', R4 , R5 orR7.

21. The fluorescent polymeric material of Claim 9 wherein the lipid soluble rhodamine dye comprises core structure (IV).

22. The fluorescent polymeric material of claim 9 wherein the lipid soluble rhodamine dye comprises core structure (IV) and further comprises one or more substituted or unsubstitated five or six membered fused rings that comprise, as part of the ring, a nitrogen atom from either the exocyclic amine or exocyclic imminium group and one of R2', R4, R5 or R7'.

23. The fluorescent polymeric material of any one of Claims 1-3 in which the polymer is formed by polymerizing one or more of the following monomers: substitated or unsubstitated styrenes, acrylate, alkyl acrylates, methacrylate, alkyl methacrylates, acrylonitrile, alkyl acrylonitrile, esters, acetates, amides, alcohols, acrolein, dimethylsiloxane, butadiene, isoprene, urethane, vinylacetate, vinylchloride, vinylpyridine, vinylbenzylchloride, vinyltoluene, vinylidene chloride and divinylbenzene, and mixtures thereof, with or without the additional presence of a crosslinking agent.

24. The fluorescent polymeric material of Claim 23 wherein the polymer is a hydrophobic copolymer of styrene, acrylic acid, and a polyvinyl crosslinking agent.

25. The fluorescent polymeric material of any one of Claims 1-4 wherein the polymer is in the form of a microparticle having a diameter in the range of about 0.01 to 1000 micrometers.

26. The fluorescent polymeric material of any one of Claims 1-4 in which the lipid-soluble rhodamine dye is incorporated into the polymer non-covalently.

27. The fluorescent polymeric material of any one of Claims 1, 3 and 4 wherein the polymer is activated so that it comprises one or more groups that have a reactive functionality.

28. The fluorescent polymeric material of any one of Claim 27 wherein the reactive functionality is an elecfrophile selected from pentafluorphenyl ester, NHS-ester, acrylamides, acyl azides, acyl halides, acyl nitriles, aldehydes or ketones, alkyl halides, alkyl sulfonates, anydrides, aryl halides, aziridines, boronates, carboxylic acids, carbodiimides, diazoalkenes, epoxides, haloacetamides, halotriazines, imido esters, isocyanates, isothiocyanates, maleimides, phorphoramidites, silyl halides, sulfonate esters, and sulfonyl halides.

29. The fluorescent polymeric material of Claim 27 wherein the reactive functionality is a nucleophile selected from amines/anilines, alcohols/phenols, thiols, hydrazines, and hydroxylamines.

30. The fluorescent polymeric material of any one of Claims 1-4 that further includes another substance that is absorbed into, adsorbed onto or covalently attached to the polymer.

31. The fluorescent polymeric material of Claim 30 in which the other substance is a biomolecule or a probe for a biomolecule.

32. The fluorescent polymeric material of Claim 31 in which the other substance is a biomolecule selected from amino acids, polypeptides, glycosolated and unglycosolated proteins, nucleosides, nucleotides, oligonucleotides, polynucleotides, nucleic acids, polynucleic acids, carbohydrates, and steroids.

33. The fluorescent polymeric material of claim 31 in which the other substance is a biomolecule selected from haptens, antibodies, enzymes, toxins and hormones.

34. The fluorescent polymeric material of claim 30 in which the other substance is selected from antigens, drugs, vitamins, poisons, and pathogens.

35. The fluorescent polymeric material of any one of Claims 1-4 which includes a mixtare of from 1 to 10 fluorescent dyes.

36. The fluorescent polymeric material of Claim 35 in which each of the fluorescent dyes is independently selected lipid soluble rhodamine dye comprising one of core structures (I), (II), (III) and (IV), acridine dyes, alizarene dyes, azo dyes, anthraquinine dyes, bodipy dyes, coumarin dyes, cyanine dyes, fluorescein dyes, lanthanide complexes, oxazine dyes, phenazathionium dyes, phenazoxonium dyes, porphyorin dyes, pyrene dyes, pyrilium dyes, perylene dyes, phenoxazine dyes, phenezine dyes, rhodol dyes and xanthene dyes.

37. The fluorescent polymeric material of Claim 35 in which all of the fluorescent dyes fluoresce when irradiated with the same excitation source.

38. The fluorescent polymer material of Claim 35 in which two or more fluorescent dyes fluoresce when irradiated with multiple excitation sources.

39. The fluorescent polymeric material of Claim 35 wherein at least one of the fluorescent dyes is an energy transfer dye.

40. A collection of fluorescent polymeric particle populations, each of which has a unique, spectrally resolvable fluorescent emission spectrum and at least one of which comprises particles that comprise a polymer having incoφorated internally therein at least one lipid-soluble rhodamine dye comprising one of the following core structures:





where R1 R2, R2', R3', R3", R4, R4', R5', R5, R6", R6', R7', R7, R8, R9, R11 R12, R13, R14 and R15 are, independently, selected from hydrogen or the same or different substitaent groups having no more than 40 atoms,

where at least one of R3', R3", R6" and R6' is a lipophilic substitaent;

where R9 in core structure (II) is a nonaromatic substitaent; and

where, with respect to core structures (I), (III) and (IV), R1 and R8 are not, simultaneously, pendant or fused benzo, naptho or polycyclic aryleno groups.

41. The collection of Claim 40 wherein at least one of R3', R3", R6" and R6' is a lipophilic substitaent selected from (C4-C20) alkyls and heteroalkyls, (C5-C20) aryls and heteroaryls, and (C6-C40) arylalkyls or heteroaryl alkyls.

42. The collection of Claim 40 wherein at least one of R3', R3", R6" and R6' is a lipophilic substitaent is selected from (C7-C20) alkyls.

43. The collection of Claim 40 wherein at least two of R3', R6", R3" and R6" are the same or different lipophilic substitaent.

44. The collection of Claim 40 wherein all of R3', R6", R3" and R6" are the same or different lipophilic substitaent.

45. The collection of claim 40 wherein the substitaents in core structures (I), (II), (III) and (IV) are further defined as follows:

(i.) with respect to core structure (I),

R1 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substituted with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substituted with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R1 is taken together with R2 form part of a benzo, naptho or polycyclic aryleno group which is optionally substituted with one or more of the same or different Ra or suitable Rb groups,

R2 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substituted with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substituted with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substituted with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R2 is taken together with R1 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R2 is taken together with R3' to form a 5- or 6-membered ring which is optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R3 is selected from hydrogen, (C4-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R3 is taken together with R2 to form a 5- or 6-membered ring which is optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R3 is selected from (C4-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R3 is taken together with R4 to form a 5- or 6-membered ring which is optionally substituted with one or more of the same or different Ra or suitable Rb groups, R4 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substituted with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substituted with one or more of the same or different Ra or suitable Rb groups, or, alternatively R4 is taken together with R3 to form a 5- or 6-membered ring which is optionally substituted with one or more of the same or different Ra or suitable Rb groups, R5 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb group or, alternatively R5 is taken together with R6 to form a 5- or 6-membered ring which is optionally substitated with one or more of the same or different Ra or suitable R groups, R6 is selected from (C4-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups or, alternatively R6" is taken together with R5 to form a 5- or 6-membered ring which is optionally substitated with one or more of the same or different Ra or suitable Rb groups, R6 is selected from hydrogen, (C4-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R6 is taken together with R7 to form a 5- or 6-membered ring optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R7 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C -C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substitated with one or more of the same or different Ra or Rb groups, or, alternatively, R7 is taken together with R6 to form a 5- or 6-membered ring optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R7 is taken together with R8 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substituted with one or more of the same or different Ra or suitable Rb groups,

R8 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R 8 together with R7 form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R11 and R15 are each, independently of one another, selected from hydrogen, halo, (C1-C20) alkyl, haloalkyl, -ORy, -SRy, -SORy, -SO2Ry, -C(O)ORy, -S(O)2ORy, amide, sulfonamide and nitrile,

R12, R13 and R1 are each, independently of one another, selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups,

Rx is selected from -NRcRc, -ORd, -SRd, halo, haloalkyl, -CN, -NC, -OCN, -SCN, -NO, -NO2, -N3, -S(O)Rd, -S(O)2Rd, -S(O)2ORd, -S(O)NRcRc, -S(O)2NRcRc, -OS(O)Rd, -OS(O)2Rd, -OS(O)2ORd, -OS(O)2NRcRc, -C(O)Rd, -C(O)ORd, -C(O)NRcRc, -C(NH)NRcRc, -OC(O)Rd, -OC(O)ORd, -OC(O)NRcRc and -OC(NH)NRcRc,

Ry is selected from (C1-C20) alkyls or heteroalkyls optionally substitated with lipophilic substitaents, (C5-C20) aryls or heteroaryls optionally substitated with lipophilic substitaents and (C6-C26) arylalkyl or heteroarylalkyls optionally substitated with lipophilic substitaents,

Ra is selected from hydrogen, (C 1 -C8) alkyl or heteroalkyl, (C5-C20) aryl or heteroaryl and (C6-C28) arylalkyl or heteroarylalkyl,

Rb is selected from -NRcRc, =O, -ORd' =S, -SRd, =NRd, =NORd, halo, haloalkyl, , -CN, -NC, -OCN, -SCN, -NO, -NO2, =N2, -N3, -S(O)Rd, -S(O)2Rd, -S(O)2ORd, -S(O)NRcRc, -S(O)2NRcRc, -OS(O)Rd, -OS(O)2Rd, -OS(O)2ORd, -OS(O)2NRcRc, -C(O)Rd, -C(O)ORd, -C(O)NRcRc, -C(NH)NRcRc, -OC(O)Rd, -OC(O)ORd, -OC(O)NRcRc and -OC(NH)NRcRc,

each Rc is independently hydrogen or Rd, or, alternatively, each Rc is taken together with the nitrogen atom to which it is bonded to form a 5 to 8-membered saturated or unsatarated ring which may optionally include one or more of the same or different additional heteroatoms and which may optionally be substituted with one or more of the same or different Ra or R groups,

each Rd is independently Ra or Ra substitated with one or more of the same or different Ra or Re groups, and

each Re is selected from -NRaRa, =O, -ORa, =S, -SRa, =NRa, =NORa, halo, haloalkyl, -CN, -NC, -OCN, -SCN, -NO, -NO2, =N2, -N3, -S(O)Ra, -S(O)2Ra, -S(O)2ORa, -S(O)NRaRa, -S(O)2NRaRa, -OS(O)Ra, -OS(O)2Ra, -OS(O)2ORa, -OS(O)2NRaRa, -C(O)Ra, -C(O)ORa, -C(O)NRaRa, -C(NH)NRaRa, -OC(O)Ra, -OC(O)ORa, -OC(O)NRaRa and -OC(NH)NRaRa; and

(ii.) with respect to core structure (II),

R1, R2, R3', R3", R4, R5, R6", R6', R7, R8, Rx, Ra, Rb, Rc, Rd, and Re are as first defined in section 5.6 with respect to core structure (I), and

R9 is selected from hydrogen, alkyl, halo, haloalkyl, or nitrile;

(iii.) with respect to core structure (III),

R1, R3", R5, R6", R6', R7, R8, R11, R12, R13, R14, R15 Rx, Ry, Ra, Rb, Rc, Rd, and Re are as first defined in section 5.6 with respect to core structure (I),

R2 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R2 is taken together with R1 or R2 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R2 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R2 is taken together with R2 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R2' is taken together with R3' to form a 5- or 6-membered ring which is optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R3' is selected from hydrogen, (C4-C20) alkyl or heteroalkyl optionally substituted with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substituted with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R3' is taken together with R2 to form a 5- or 6-membered ring which is optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R4 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substituted with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively R4 is taken together with R3 to form a 5- or 6-membered ring which is optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R4 is taken together with R4 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups, and

R4' is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substituted with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively R4 is taken together R4 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups; and

(iv.) with respect to core structure (IV),

R1, R3", R6", R8, R11, R12, R13, R14, R15 Rx, Ry, Ra, Rb, Rc, Rd, and Re are as first defined in section 5.6 with respect to core structure (I),

R2, R2', R3 ', R4, and R4' are as defined in section 5.8 with respect to core structure (III),

R5' is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb group or, alternatively R5 is taken together with R5 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R5 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb group or, alternatively R5 is taken together with R6 to form a 5- or 6-membered ring which is optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R5 is taken together with R5 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups,

R6 is selected from hydrogen, (C4-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroarylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R6 is taken together with R7 to form a 5- or 6-membered ring optionally substituted with one or more of the same or different Ra or suitable Rb groups,

R7' is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substituted with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substituted with one or more of the same or different Ra or Rb groups, or,

alternatively, R7 ' is taken together with R6' to form a 5- or 6-membered ring optionally substitated with one or more of the same or different Ra or suitable Rb groups, or, alternatively, R7' is taken together with R7 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups, and

R7 is selected from hydrogen, Rx, (C1-C20) alkyl or heteroalkyl optionally substituted with one or more of the same or different Rb groups, (C5-C20) aryl or heteroaryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl or heteroaryl alkyl optionally substituted with one or more of the same or different Ra or Rb groups, or, alternatively, R7 is taken together with R7' or R8 to form part of a benzo, naptho or polycyclic aryleno group which is optionally substitated with one or more of the same or different Ra or suitable Rb groups.

46. The collection of Claim 40 wherein the lipid soluble rhodamine dye comprises core structure (I).

47. The collection of Claim 40 wherein the lipid soluble rhodamine dye comprises core structure (I) and, additionally, the substitaents therein comprise one or more of the following features:

R1 and R8 are each, independently of one another, selected from hydrogen, (C1-C8) alkyl, halo, and -CF3, or are part of a fused aryl or heteroaryl group with R2 and R7, respectively;

R2 and R7 are each, independently of one another, selected from hydrogen, (C1-C8) alkyl, halo, -CF3, -CN, carboxylate, ester, sulfone, amino, amido and oxoether, or are part of a fused aryl or heteroaryl group with R1 and R8, respectively, or are part of a 5 or 6 membered ring with R3 and R6 , respectively;

R3' and R6' are each, independently of one another, selected from hydrogen and (C4-C20) alkyl, or are part of a 5 or 6 membered ring with R2 and R7, respectively;

R3" and R6" are each, independently of one another, selected from hydrogen and (C4-C20) alkyl or are part of a 5 or 6 membered ring with R4 and R5, respectively;

at least two of R3', R3", R6', R6" are not hydrogen;

R4 and R5 are each, independently of one another, selected from hydrogen, (C1-C8) alkyl, halo, -CF3, aryl, heteroaryl, -CN, carboxylate, ester, and sulfone, or are part of a 5 or 6 membered ring with R3 and R6 , respectively;

R11 and R15 are each, independently, selected from hydrogen, halo, ester, amide, sulfonamide, (C 1 -C20) alkyl, -ORe, -SRe, -CN and sulfone; and

R12, R13 and R14 are each, independently of one another, selected from hydrogen, halo, alkyl, aryl, (C4 to C20) alkyl ester, sulfone, -CF3 and -CN.

48. The collection of Claim 40 wherein the lipid soluble rhodamine dye comprises core structure (I) and, additionally, the substitaents therein comprise one or more of the following featares:

R1 and R8 are each, independently of one another, selected from hydrogen, (C1-C8) alkyl, and halo, or are part of a fused aryl or heteroaryl group with R2 and R8, respectively;

R2 and R7 are each, independently of one another, selected from hydrogen, (C1-C8) alkyl, and halo, or are part of a fused aryl or heteroaryl group with R1 and R8 respectively, or are part of a 5 or 6 membered ring with R3 and R6 , respectively;

R3' and R6' are each the same or different (C4-C20) alkyl;

R3 and R6 are each, independently of one another, selected from hydrogen and the same or different (C4-C20) alkyl, or are part of a 5 or 6 membered ring with R4 and R5, respectively;

R4 and R5 are each, independently of one another, selected from hydrogen, (C1-C8) alkyl, aryl and heteroaryl and halo, or are part of a 5 or 6 membered ring with R3 and R6 , respectively;

R11 and R15, independently of one another, selected from hydrogen, fluoro, chloro, (C 1 -C20) alkyl, -ORe and -SRe; and

R12, R13 and R14 are each, independently of one another, selected from hydrogen and halo.

49. The collection of claim 40 wherein the lipid soluble rhodamine dye comprises one of the following structural embodiments of core structure (I):











where each Rz is independently selected from hydrogen, Rb, (C1-C20) alkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl optionally substitated with one or more of the same or different Ra or suitable R group; and

R1, R2, R3', R3", R4, R5, R6', R6", R7, R8, R11, R12, R13, R14, R15, Ra and Rb are as previously defined.

50. The collection of Claim 40 wherein the lipid soluble rhodamine dye is one of the following embodiments of core structure (I):











51. The collection of Claim 40 wherein the lipid soluble rhodamine dye comprises core structure (II).

52. The collection of Claim 40 wherein the lipid soluble rhodamine dye comprises core structure (II) and, additionally, the substitaents therein comprise one or more of the following featares:

R1 when taken together with R2 forms part of a fused benzo, naptho or polycyclic aryleno group that is optionally substitated with one or more of the same or different Ra or suitable R groups; and

R7 when taken together with R8 forms part of a fused benzo, naptho or polycyclic aryleno group that is optionally substituted with one or more of the same or different Ra or suitable Rb groups.

53. The collection of Claim 40 wherein the lipid soluble rhodamine dye comprises one of the following structural embodiments of core structure (II):





wherein each RQ is independently selected from hydrogen, (C1-C20) alkyl optionally substitated with one or more of the same or different Rb groups, (C5-C20) aryl optionally substitated with one or more of the same or different Ra or suitable Rb groups and (C6-C40) arylalkyl optionally substitated with one or more of the same or different Ra or suitable Rb group; and Raand Rb are as previously defined.

54. The collection of Claim 40 wherein the lipid soluble rhodamine dye is one of the following embodiments of core structure (II):





55. The collection of Claim 40 wherein the lipid soluble rhodamine dye comprises core structure (III).

56. The collection of claim 40 wherein the lipid soluble rhodamine dye comprises core structure (III) and further comprises one or more substitated or unsubstituted five or six membered fused rings that comprise, as part of the ring, a nitrogen atom from either the exocyclic amine or exocyclic imminium group and one of R2', R4, R5 or R7.

57. The collection of Claim 40 wherein the lipid soluble rhodamine dye comprises core structure (IV).

58. The collection of claim 40 wherein the lipid soluble rhodamine dye comprises core structure (IV) and further comprises one or more substitated or unsubstituted five or six membered fused rings that comprise, as part of the ring, a nitrogen atom from either the exocyclic amine or exocyclic imminium group and one of R2', R4, R5 or R7'.

59. The collection of Claim 40 in which the polymer is formed from one or more of the following monomers: substitated or unsubstitated styrenes, acrylate, alkyl acrylates, methacrylate, alkyl methacrylates, acrylonitrile, alkyl acrylonitrile, esters, acetates, amides, alcohols, acrolein, dimethylsiloxane, butadiene, isoprene, urethane, vinylacetate, vinylchloride, vinylpyridine, vinylbenzylchloride, vinyltoluene, vinylidene chloride and divinylbenzene, and mixtures thereof, with or without the additional presence of a crosslinking agent.

60. The collection of Claim 59 in which the polymer is a hydrophobic copolymer of styrene, acrylic acid, and a polyvinyl crosslinking agent.

61. The collection of Claim 40 in which each of the particles comprising the lipid soluble rhodamine dye is a microparticle having a diameter in the range of about 0.01 to 1000 micrometers.

62. The collection of Claim 40 in which the lipid-soluble rhodamine dye is incoφorated into the polymer non-covalently.

63. The collection of Claim 40 in which one or more of the particles comprising the lipid soluble rhodamine dye is activated to comprise one or more groups that have a reactive functionality.

64. The collection of Claim 63 in which the reactive functionality is an elecfrophile selected from pentafluorphenyl ester, NHS-ester, acrylamides, acyl azides, acyl halides, acyl nitriles, aldehydes or ketones, alkyl halides, alkyl sulfonates, anydrides, aryl halides, aziridines, boronates, carboxylic acids, carbodiimides, diazoalkenes,

epoxides, haloacetamides, halotriazines, imido esters, isocyanates, isothiocyanates, maleimides, phorphoramidites, silyl halides, sulfonate esters, and sulfonyl halides.

65. The collection of Claim 63 in which the reactive functionality is a nucleophile selected from amines/anilines, alcohols/phenols, thiols, hydrazines, and hydroxylamines.

66. The collection of Claim 40 in which the particles comprising the lipid soluble rhodamine dye further include another substance that is absorbed into, adsorbed onto or covalently attached to the particles.

67. The collection of Claim 66 in which the other substance is a biomolecule or a probe for a biomolecule.

68. The collection of Claim 67 in which the other substance is a biomolecule selected from amino acids, polypeptides, glycosolated and unglycosolated proteins, nucleosides, nucleotides, oligonucleotides, polynucleotides, nucleic acids, polynucleic acids, carbohydrates, and steroids.

69. The collection of Claim 67 in which the other substance is a biomolecule selected from haptens, antibodies, enzymes, toxins and hormones.

70. The collection of Claim 66 in which the other substance is selected from antigens, drugs, vitamins, poisons, and pathogens.

71. The collection of Claim 40 in which at least one fluorescent dye in the particles of each particle population is a lipid-soluble rhodamine comprising one of structures (I), (II), (III) and (IV).

72. The collection of Claim 40 in which each particle population fluoresces when illuminated by the same excitation source.

73. The collection of claim 40 in which two or more fluorescent dyes fluoresce when irradiated with multiple excitation sources.

74. The collection of Claim 40 in which at least one of the particle populations comprises a plurality of fluorescent dyes.

75. The collection of Claim 40 in which the fluorescent dyes of the plurality are each, independently of one another, selected from lipid soluble rhodamine dye comprising structures (I), (II), (III) or (IV), acridine dyes, alizarene dyes, azo dyes, anthraquinine dyes, bodipy dyes, coumarin dyes, cyanine dyes, fluorescein dyes, lanthanide complexes, oxazine dyes, phenazathionium dyes, phenazoxonium dyes, porphyorin dyes, pyrene dyes, pyrilium dyes, perylene dyes, phenoxazine dyes, phenezine dyes, rhodol dyes and xanthene dyes.

76. The collection of Claim 40 wherein the particles comprising the lipid soluble rhodamine additionally comprise an energy transfer dye.

77. A fluorescent polymeric material comprising a polymer and an energy transfer dye comprising two to four covalently attached dyes wherein one of said covalently attached dyes is a lipid soluble rhodamine dye that comprises one of the following "core" structures:

where at least one of R3', R3", R6" and R6' is a lipophilic substitaent;

where R9 in core structure (II) is a nonaromatic substitaent;

where, with respect to core structures (I), (III) and (IV), R1 and R8 are not, simultaneously, pendant or fused benzo, naptho or polycyclic aryleno groups;

where at least one of R2, R2', R3', R4, R4', R5', R5, R6', R7', R7, R12, R13, and R14 corresponds to the moiety -S^LK-S^D, where D is an acceptor or donor dye, S1 and S2 are, individually, a covalent bond or a spacing moiety selected from substitated or unsubstitated alkyls, heteroalkyls, aryls and heteroaryls containing no more than 20 atoms, and LK is any reacted linking group; and

where the remainder of R1 R2, R2', R3', R3", R4, R4', R5', R5, R6", R6', R7', R7, R8, R9, R11 R12, R13, R14 and R15 are, independently, selected from hydrogen or the same or different substituent groups having no more than 40 atoms.

78. The fluorescent polymeric material of claim 77 wherein LK is selected from an ester, an amide, a sulfonamide, a hydrazine, an imine, a maleimide, a sulfide, a disulfide, a carbamate and a thiocarbamate.

79. The fluorescent polymeric material of claim 77 wherein all of the attached dyes are lipid soluble rhodamines.

80. A fluorescent polymeric material comprising a polymeric particle and a lipid soluble rhodamine.