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1. WO2003003816 - COMPOSITIONS ET PROCEDES DESTINES A AUGMENTER LA SEGREGATION DE TRANSGENES DANS LES PLANTES

Numéro de publication WO/2003/003816
Date de publication 16.01.2003
N° de la demande internationale PCT/US2002/021350
Date du dépôt international 05.07.2002
Demande présentée en vertu du Chapitre 2 06.07.2002
CIB
C12N 15/82 2006.01
CCHIMIE; MÉTALLURGIE
12BIOCHIMIE; BIÈRE; SPIRITUEUX; VIN; VINAIGRE; MICROBIOLOGIE; ENZYMOLOGIE; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE
NMICRO-ORGANISMES OU ENZYMES; COMPOSITIONS LES CONTENANT; CULTURE OU CONSERVATION DE MICRO-ORGANISMES; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE; MILIEUX DE CULTURE
15Techniques de mutation ou génie génétique; ADN ou ARN concernant le génie génétique, vecteurs, p.ex. plasmides, ou leur isolement, leur préparation ou leur purification; Utilisation d'hôtes pour ceux-ci
09Technologie d'ADN recombinant
63Introduction de matériel génétique étranger utilisant des vecteurs; Vecteurs; Utilisation d'hôtes pour ceux-ci; Régulation de l'expression
79Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes
82pour cellules végétales
CPC
C12N 15/8205
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
8202by biological means, e.g. cell mediated or natural vector
8205Agrobacterium mediated transformation
C12N 15/8209
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
C12N 15/8263
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8261with agronomic (input) traits, e.g. crop yield
8262involving plant development
8263Ablation; Apoptosis
C12N 15/8265
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8261with agronomic (input) traits, e.g. crop yield
8262involving plant development
8265Transgene containment, e.g. gene dispersal
C12N 15/8289
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8261with agronomic (input) traits, e.g. crop yield
8287for fertility modification, e.g. apomixis
8289Male sterility
Déposants
  • MONSANTO TECHNOLOGY LLC [US/US]; Patent Department 800 N. Lindbergh St. Louis, MO 63167, US
Inventeurs
  • BARTON, Kenneth; US
  • CROW, Laura; US
  • GILBERTSON, Larry; US
  • HUANG, Yong; US
  • MARTINELL, Brian; US
  • PETERSEN, Michael, W.; US
  • PILLER, Kenneth, J.; US
  • WANG, Qi; US
Données relatives à la priorité
60/303,37506.07.2001US
Langue de publication anglais (EN)
Langue de dépôt anglais (EN)
États désignés
Titre
(EN) METHODS FOR ENHANCING SEGREGATION OF TRANSGENES IN PLANTS AND COMPOSITIONS THEREOF
(FR) COMPOSITIONS ET PROCEDES DESTINES A AUGMENTER LA SEGREGATION DE TRANSGENES DANS LES PLANTES
Abrégé
(EN)
The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprise transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independent transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
(FR)
L'invention a trait à des compositions et procédés destinés à augmenter la sélection de plantes transgéniques comprenant deux molécules d'ADN-T intégrées dans le génome d'une plante à des loci génétiques et physiques différents. Les compositions sont des constructions d'ADN qui comprennent de nouveaux agencements de molécules d'ADN-T contenant des gènes d'intérêt, des gènes marqueurs à sélection positive, et des gènes létaux conditionnels. Les procédés décrits consistent à transformer une cellule végétale afin qu'elle comprenne les constructions d'ADN de l'invention, à régénérer la cellule végétale en une plante et à identifier des loci de transgènes indépendants, où les gènes marqueurs sélectionnables ou bien les éléments transgéniques peuvent être séparés dans les descendants.
Également publié en tant que
RU2004112785
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