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1. WO2002090443 - SYNTHESE ET EVALUATION DE NOUVEAUX COLORANTS DE CYANINE EN TANT QUE PETIT SILLON DE LIANTS DE [POLY(DA-DT)]¿2?

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

[ EN ]

CLAIMS.

1. A cyanine dye binding in the groove of DNA, selected from the group of


or


wherein A1 and A2 are each independently O, S, or N, and R is H or a carbohydrate that may contain a hetero atom, and m is 0 to 5, and n is 0 to 5.

2. A cyanine dye according to claim 1, wherein R is methyl, or ethyl, and m is 1 and n is 0.

3. A cyanine dye according to claim 1, wherein R is methyl, or ethyl, and m is 1 and n is 0 and A1 and A2 is S.

4. A cyanine dye according to claim 1, wherein R is methyl, or ethyl, and m is 1 and n is 0 and A1 and A2 is O.

5. A cyanine dye according to claim 1, wherein R is methyl, or ethyl, and m is 1 and n is 0 and A1 is S and A2 is O.

3. A cyanine dye according to claim 1, having the pyridine/quinoline ring in 2- position.

4. Probe for nucleic acid hybridization comprising a cyanine dye according to claims

1-2.

5. Method for carrying out a real-time PCR-reaction of a DNA template, wherein a fluorescent dye increasing its fluorescent reaction when it is looked in a minor groove position in a double stranded DNA is used, whereby the dye comprises at least 2 aromatic ring systems both comprising at least one nitrogen atom, which rings are linked by a alkine goup having up to four carbon atoms to form a conjugated bond, and the dye further comprises at least a third aromatic system linked thereto via a bond having a significant double string character, such as a single bond or a ethin bond, to provide a stiff conjugated system.

6. Method according to claim 4, wherein the dye is an asymmetric cyanine dye.

7. Method according to claim 4, wherein one of the cyanine residues contains S and/or O.

8. Method according to claim 4, wherein the dye compound is crescent shaped.

9. Method according to claim 4, wherein the dye is a derivative according to one or more of claims 1-2.