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1. WO1999000673 - TECHNIQUES DIAGNOSTIQUES UTILISANT LE RECEPTEUR SOLUBLE DE PROTEINE C/PROTEINE C ACTIVEE DE CELLULES ENDOTHELIALES

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[ EN ]

We claim:
1. An assay for soluble endothelial protein C receptor comprising
obtaining a sample from a patient to be tested, and
measuring the amount of soluble endothelial protein C receptor.
2. The assay of claim 1 wherein the sample is selected from the group consisting of urine, plasma, serum, tissue samples, and interstitial fluid.
3. The assay of claim 1 further comprising the step of correlating the amount of soluble endothelial protein C receptor with calibration standards.
4. The assay of claim 1 wherein the patient has a disorder or disease selected from the group consisting of coagulation and
inflammatory states and disorders, disorders or diseases involving damage to endothelium, and large blood vessel disease.
5. The assay of claim 4 wherein the disorder or disease is selected from the group consisting of autoimmune diseases,
transplantation, sepsis, shock, pre-eclampsia, diabetes, vascular disease, kidney disease and liver disease.
6. The assay of claim 5 wherein the vascular disease is selected from the group consisting of cardiopulmonary bypass, unstable angina, restenosis, and angioplasty.
7. The assay of claim 1 to detect damage to large blood vessels.

8. A kit for detection and measurement of endothelial protein C receptor comprising
an antibody immunoreactive with endothelial protein C receptor,
reagents to detect a reaction between the antibody and endothelial protein C receptor in a sample from a patient, and
standards to correlate the amount of reaction to normal and abnormal levels of endothelial protein C receptor.
9. The kit of claim 8 where the antibody has a higher affinity for endothelial protein C receptor including the transmembrane domain than for endothelial protein C receptor not including the transmembrane domain.
10. The kit of claim 8 wherein the antibody is immunoreactive with the insert in an alternatively spliced endothelial protein C receptor.

11. The kit of claim 8 wherein teh antibodies block binding of endothelial protein C receptor and activated protein C or protein C.
12. A modified endothelial protein C receptor wherein the carboxyl terminal cysteine residue is replaced with another amino acid or is not palmitoylated.
13. A modified endothelial protein C receptor which is not gly cosy lated.
14. An isolated alternatively spliced endothelial protein C receptor.
15. An isolated soluble endothelial protein C receptor cleaved at a naturally occuring proteolytic cleavage site before the
transmembrane domain.