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1. WO1991016423 - ENZYMES POUR DETERGENTS RESISTANTES A L'OXYDATION

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CLAIMS :

1. Oxidation stable chemically modified detergent enzymes, wherein one or more methionins have been mutated into cysteins, characterized in, that the cysteins have been chemically modified in order to substitute the hydrogen of the HS-group into a group of the general formula R1S-, wherein R1 is C1.6-alkyl.

2. Oxidation stable chemically modified detergent enzymes, according to claim 1, characterized in, that the detergent enzymes are amylases, lipases, cellulases or proteases.

3. Oxidation stable chemically modified detergent enzymes according to claim 1, characterised in, that the detergent enzymes are subtilisins.

4. Oxidation stable detergent enzymes according to any of claims 1-3, characterized in, that R1 is C^-alkyl.

5. Oxidation stable chemically modified subtilisins, wherein the methionine in position 222, adjacent to the active serine, has been mutated into cysteine, characterized in, that the cysteine has been chemically modified in order to substitute the hydrogen of the HS-group into a group of the general formula R1S-, wherein R1 is C^-alkyl.

6. Oxidation stable chemically modified subtilisin according to claim 5, characterized in, that the subtilisin is subtilisin-309, subtilisin-147 , subtilisin BPN', subtilisin Carlsberg, or proteinase K.

7. Oxidation stable chemically modified subtilisin according to claim 5, characterized in, that the subtilisin is subtilisin-309.

8. Oxidation stable chemically modified subtilisin according to claims 5-7, characterized in, that R1 is c,_3- alkyl.

9. Oxidation stable chemically modified subtilisin, Subtilisin-309-M222C-SM.

10. Oxidation stable chemically modified subtilisin, Subtilisin-309-M222C-SP.

11. A process for stabilizing detergent enzymes against oxidation, characterized in, that a variant of the detergent enzyme, wherein one or more methionins have been mutated into cysteins, are chemically modified by substitution of the hydrogen of the HS-group of the cysteins into a group of the general formula R1S-, wherein R1 is C^-alkyl, due to reaction with a compound of the general formula R1SS02R2, wherein R1 is C^-alkyl, and R2 is C^-alkyl, and the reaction is carried out at a pH-value in the range 5 to 11.

12. A process for stabilizing detergent enzymes against oxidation, according to claim 11, characterized in, that the detergent enzymes are amylases, lipases, cellulases or proteases.

13. A process for stabilizing subtilisins against oxidation, characterised in, that subtilisin variants, wherein one or more methionins have been mutated into cysteins, are chemically modified by substituting the hydrogen of the HS-group of the cysteins into a group of the general formula R1S-wherein R' is C^-alkyl, due to reaction with a compound of the general formula R1SS02R2, wherein R1 is C1.6-alkyl, and R2 is C1.4-alkyl, and the reaction is carried out at a pH-value in the range 5 to 11.

14. A process according to claim 13, characterized in, that the reaction is carried out at a pH-value in the range 8 to 10.

15. A process according to either of claims 13-14, characterized in, that R1 is G,_6-alkyl, and R2 is C,_2-alkyl.

16. A process according to either of claims 13-14, characterized in, that R is C1.3-alkyl, and R2 is C1.2-alkyl.

17. A detergent composition comprising an oxidation stable detergent enzyme according to any of claims 1-10.

18. A detergent composition according to claim 17, which further comprises one or more other enzymes, in particular amylases; lipases; cellulases; or peroxidases.

19. A detergent composition comprising an oxidation stable detergent enzyme according to any of claims 1-10, provided in the form of a detergent additive, preferably a non-dusting granulate, a liquid, in particular a stabilized liquid, a slurry, or a protected enzyme.