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1. WO1988003956 - PROCEDE POUR DETECTER UN VIRUS DANS UNE PLANTE

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

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CLAIMS

1. A process for detecting plant virus, c h a r a c t e r i s e d in that plant sap is treated with a protein-decomposing enzyme for removal of endogenously bound biotin, that virus RNA from said plant sap is bound to a filter membrane, and that prehybridisation is carried out, whereupon virus RNA is hybridised by incubation of the membrane with biotin-labelled DNA which is homologous to RNA in the virus to be detected, the presence of the specific virus RNA becoming visually detectable through the occurrence of a colour after addition of a biotin-specific enzyme.
2. A process as claimed in claim 1, c h a r a c t e r i s e d in that said plant sap is recovered from potato leaves or tubers, and that said plant virus is potato virus Y, X, S, A or M.
3. A process as claimed in claim 1, c h a r a c t e r i s e d in that said plant sap is recovered from pasture grass or corn, and that said plant virus is barley yellow dwarf virus.

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