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1. (WO1979000045) PROCEDE DE DETERMINATION DE L"ACTIVITE ENZYMATIQUE PAR DIFFUSION DANS UNE MATRICE POREUSE OU PAR ELECTROPHORESE
Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

CLAIMS:¬

1. A method of determining enzymatic activity, characterized in that there is deposited on a solid surface a thin layer of a substance which is sensitive to enzymatic lysis, whereafter an immobilized matrix is applied to the thin layer and in that there is supplied to the immobilized matrix a quantity of an enzyme-containing sample, which is permitted to diffuse or migrate electrophoretically in the matrix and react with the substance sensitive to enzymatic lysis, whereafter the matrix is removed for allowing the lysis to be indicated on the solid surface.
2. A method according to claim 1, characterized in that extra-cellular enzymes formed upon bacterial growth are permitted to diffuse through an immobilized matrix comprising a nutrient for bacterial growth, and in that a lysis of the substance deposited on the solid surface is allowed to be indicated subsequent to removing the matrix.
3. A method according to claim 1, characterized in that the solid surface constitutes a plastics material selected from the group polystyrene, polyacrylnitrile, polyolefines and copolyme thereof.